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In the context of ribosomes during protein synthesis, the P site (peptidyl site) and A site (aminoacyl site) are two key binding sites for tRNA. The A site is where the aminoacyl-tRNA, carrying the next amino acid to be added, binds during translation. The P site holds the tRNA that carries the growing polypeptide chain. This coordinated action facilitates the addition of amino acids to the growing protein chain during translation.

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