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3-5 years
enzymes known as restriction endonucleases. These enzymes recognize specific nucleotide sequences and cleave the DNA at those sites. This process is often used in molecular biology for tasks such as gene cloning and DNA sequencing.
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Restriction enzymes recognize specific sequences of nucleotides in DNA molecules, called recognition sites. These enzymes then bind to these sites and cut the DNA at specific points within or near the recognition site, resulting in the cleavage of the DNA molecule.
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Restriction enzymes recognize specific DNA sequences known as recognition sites, which are typically palindromic and range in length from 4 to 8 base pairs. These enzymes can cleave DNA at these recognition sites, either by cutting between specific bases within the recognition sequence or nearby.
A restriction enzyme is a type of endonuclease. Endonucleases are enzymes that cut DNA at specific sequences, while restriction enzymes specifically cut DNA at recognition sites called restriction sites.
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Produce a cut (usually staggered) at a specific recognition sequences on DNA.
Restriction enzymes cut DNA at specific sites called restriction sites. These restriction sites are typically 6 - 8 nucleotides in length and have a defined set of nucleotide bases. For example, the restriction enzyme Eco R1 cuts at the site: AGGTTC. Therefore, if the target DNA contains the above sequence, Eco R1 is able to cut it within the restriction site. Hence, by looking into the target site and which restriction enzymes are being used, on can make an accurate estimate of where the target DNA will be cut
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