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If you are incubating eggs hoping for chicks. Here are a few things that will cause the embryo to die in the shell.

Temperature extremes, too high or too low and the embryo will die.

Shaking and bouncing, the embryo is only lightly attached to the yolk and any extra movement can cause it to come loose and die.

Humidity too high or too low.

Not turning the eggs - they need to be turned at the minimum 3 times per day, more is better, so that the embryo does not stick to the shell.

Dirty and or cracked shells, your embryo may start growing but the dirt and cracks will let bad germs in and cause the embryo to die.

There are plenty more reasons, this is all I could think of right now.

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17y ago

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