Within the thyakoid membrane, electrons from water are "excited" by photons of light energy in Photosystem II. The excited electrons "fall" from Photosystem II, pass through the electron transport chain (ETC) and flow into Photosystem I. As the electrons travel down the ETC, one molecole of hydrogen is pumped across the membrane from the stroma (fluid space inside the chloroplast) into the thylakoid where a higher gradient of H+. The ions pass onto the protien, ATP synthase which takes one H+ ion and pumps it through the membrane acting like a motor generating one molecule of ATP. The ATP is now located in the stroma and will be used shortly in the Calvin Cycle.
A proton gradient is generated when protons leave a thylakoid via ATP-synthase. It works in conjunction with the electron transport and ATP synthesis during cellular respiration.
hydrogen ion enters
ATP.
Hydrogen ion leaves the thylakoid lumen.
NH3 enters the thylakoid lumen and eats the protons, by binding them it and decreases the proton gradient compared to the outside of the lumen. This "uncouples" the proton motive and reduces ATP synthesis
Chemiosmosis in the thylakoid membrane results in the synthesis of ATP during light reaction. Thylakoid membranes contain proteins. These proteins make use of light energy to drive electron transport chains. This generates a chemiosmotic potential across the thylakoid membrane and NADPH. The ATP synthase enzyme uses the chemiosmotic potential to make ATP during photo- phosphorylation.
ATP synthase complex
A high concentration of H in the thylakoid compartment provides energy for the production of ATP and ATP synthase. ATP is responsible for the transportation of chemical energy within cells, which is necessary for metabolism.
Hydrogen ion leaves the thylakoid lumen.
NH3 enters the thylakoid lumen and eats the protons, by binding them it and decreases the proton gradient compared to the outside of the lumen. This "uncouples" the proton motive and reduces ATP synthesis
The light dependent reactions take place in the thylakoid of the chloroplast. ATP is formed in the ATP synthase protein by the assistance of the hydrogen gradient produced in the electron transport chain.
ATP produced by noncyclic flow electrons in thylakoid membrane.
The pigment molecules and electron transport chains involved in the light-dependent reactions of photosynthesis are embedded in the thylakoid membrane. As energy is released from electrons traveling through the chain of acceptors, it is used to pump protons (that is, H+ ions) from the stroma of the chloroplast across the thylakoid membrane and into the center of the thylakoid. Thus, protons accumlate within the thylakoids, lowering the pH of the thylakoid interior and making it more acidic. A proton gradient possesses potential energy that can be used to form ATP.Protons are prevented from diffusing out of the thylakoid because the thylakoid membrane is impermeable to protons except at certain points bridged by an enzyme called ATP synthase. This protein extends across the thylakoid membrane and forms a channel through which protons can leave the thylakoid. As the protons pass through ATP synthetase, energy is released, and this energy is tapped by ATP synthase to form ATP from ADP and inorganic phosphate. The coupling of ATP synthesis to a protein gradient formed by energy released during electron transport is called chemiosmosis.
Protons produced from the oxygen evolving complex and the cytochrome b6f complex in photosynthesis produce a proton pool or gradient in the thylakoid lumen. These protons then movie through ATP synthase to produce ATP from ADP + Pi. This ATP is later used in conjunction with NADPH to power the Calvin Cycle.
The pigment molecules and electron transport chains involved in the light-dependent reactions of photosynthesis are embedded in the thylakoid membrane. As energy is released from electrons traveling through the chain of acceptors, it is used to pump protons (that is, H+ ions) from the stroma of the chloroplast across the thylakoid membrane and into the center of the thylakoid. Thus, protons accumlate within the thylakoids, lowering the pH of the thylakoid interior and making it more acidic. A proton gradient possesses potential energy that can be used to form ATP.Protons are prevented from diffusing out of the thylakoid because the thylakoid membrane is impermeable to protons except at certain points bridged by an enzyme called ATP synthase. This protein extends across the thylakoid membrane and forms a channel through which protons can leave the thylakoid. As the protons pass through ATP synthetase, energy is released, and this energy is tapped by ATP synthase to form ATP from ADP and inorganic phosphate. The coupling of ATP synthesis to a protein gradient formed by energy released during electron transport is called chemiosmosis.
ATP
ATP.
The photons for sunlight will not transfer energy to the electrons in photosystem 1 & 2, leading to not enough energy for the ETC to pump H+ into the lumen of the thylakoid and produce ATP for the Calvin cycle.
where does the energy used to establish the proton gradient across the thylakoid membrane come from? In other words, from splitting of water. well that's not what he said but there you go.
Chemiosmosis in the thylakoid membrane results in the synthesis of ATP during light reaction. Thylakoid membranes contain proteins. These proteins make use of light energy to drive electron transport chains. This generates a chemiosmotic potential across the thylakoid membrane and NADPH. The ATP synthase enzyme uses the chemiosmotic potential to make ATP during photo- phosphorylation.