0
Preparation
0.1MCitrate Buffer
1. Add approximately 50 mL of distilled water to a
100ml volumetric flask on a magnetic stirrer.
2. Add and dissolve 1.4705gm of Sodium citrate.
3. Then with the help of Citric Acid maintain the pH of the solution upto 4.5.
Wiki User
∙ 13y ago
Wiki User
∙ 15y agoYou have to simply dissolve streptozotocin (STZ) in the freshly prepared citrate buffer pH 4.5. But first you have to calculate the amount of STZ and the volume of citrate buffer required. Here's an example for a rat weighing 150 gms. STZgiven at 45 mg/kg of body weight. So for 150 gms you need 6.75 mg of STZ. Now you need to inject this in the abdominal cavity of rat so prefer to use minimum volume of buffer. So here, we'll dissolve 9 mg of STZ in 1 ml of Citrate buffer pH 4.5 and inject 0.75 ml of this solution into rat. Citrate buffer as in British Pharmacopeia It would be better if STZ is dissolved just before use.
Wiki User
∙ 9y agoTo make one liter of buffer, weigh out point one mole of citric acid. Dissolve it in about six hundred milliliters of water. Adjust the pH with six M NaOH until the pH is four point five. Bring the volume of the solution to one liter with water.
Wiki User
∙ 10y agoTo make a citrate buffer Mix 21 g citric acid in 1 L distilled water, and 29.4 g sodium citrate in 1 L distilled water. If you only have citric acid, mix 2.1 g in just under 1 L distilled water. You can adjust the PH level by adding 1M sodium hydroxide while gently stirring the solution using a magnetic stirrer.
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How does magnesium citrate work?
Magnesium is the pure element, however it is not found in nature by itself, it always bonds with another element. So you have many forms of magnesium such as magnesium Aspartate, bicarbonate, Carbonate, Chloride, Citrate, Gluconate, Glycinate, Hydroxide, Lactate, Malate, Orotate, Oxide, Phophate, Picolinate, Propionate, Stearate, Sulfate and Taurate, to name a few. All these different forms have one other element that they are bonded with magnesium + citric acid = magnesium citrate for instance. These different forms of magnesium have two specific qualities A. they have percentage of actual elemental magnesium i.e. how much of the combination is actual pure magnesium e.g. magnesium carbonate is 45% actual magnesium, where as magnesium sulfate is only 10% actual magnesium B. they have a rough percentage of bioavailability (how absorbable and thus useable they are for the body). For instance magnesium citrate is 90% bioavailable and so most of it could be potentially used and absorbed by the body. Magnesium oxide for instance is only 4% bioavailable for the body and so if one took magnesium oxide which had 100mg of actual magnesium in it, there would only be 4mg that the body could possible use. Let me know if this answers your question. Kind Regards, Fifty Pence
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What is the mass in grams of 45 ml of water?
45mL of plain water has a mass of about 45 grams.
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Upon swallowing such a solution, you would collapse almost immediately and lose consciousness within 10-20 seconds. Death would follow in a matter of minutes. With such a massive dose taken, any medical assistance, no matter how aggressive, would prove futile. A gram mixed in with about 4 to 8 oz water would render you unconscious within 15-45 sec (depending on your body) and without medical attention you would be dead within 30-45 min.
How many protons electrons and neutrons does an atom with atomic number 20 and a mass number 45 contain?
Atomic number 20 therefore 20 protons and 20 electrons. Mass number 45 therefore 45-20 = 25 neutrons.
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45.
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What does a stp s-45 oil filter fit
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Kidnaping case 45 years 2 life, sentenced In 01 or 02
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Latitude: 37°45′35″NLongitude: 100°01′06″W
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53°45′N 071°59′W
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because jodhpur has lotitude of 53'25 whereas delhi is in 45"01
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Sunset at Palm Springs CA on 20 Sep is around 6:45 PM PDT (21 Sep @ 01:45 UTC).
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The US would have invaded Japan on 01 Nov 45 Operation: DOWNFALL.
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10:45 - 01:40 = 09:05 five past nine
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Sunrise at Junagadh on 17 Oct 1994 was at 06:45 INST (01:15 UTC).
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orissa is the largest bauxite producing state in india with 45% of the country's total production in 200-01
What would have happened if the US had not invented the atomic bomb?
The US would have invaded Japan on 01 Nov 45 Operation: DOWNFALL and fought until victory.
