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How much SUMO1-Antibody should be used in immunoprecipitation using Protein AG to collect sumoylated proteins?
Nthu
You should start with 1 or 3 micrograms of antibody.
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∙ 16y ago
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What is the definition of the term immuniprecipitation?
The term immunoprecipitation refers to the technique of precipitating protein antigens out of a solution using an antibody that is designed to bind itself to that particular protein.
What methods can be used to study protein-protein interactions?
There are numerous methods for protein protein interactions. Biochemical methods include: 1) Coimmunoprecipitation - proteins are isolated with specific antobody. 2) Affinity Electrophoresis- estimates protein binding. 3) cross linking - to fix protein interactions before isolating 4) Quantitative immunoprecipitation: to detect interactions between endogenous non tagged proteins. Molecular Biology methods: 1) protein probing 2) two hybid system 3) phage display Genetic methods: 1) Isolation of extragenetic suppressors ,Synthetic mutants
What compound is important in cell repair?
protein
Embedded in the phospholipid bilayer?
Protein Molecules
Are proteins a enzymes?
Proteins are not enzymes. Enzymes are protein,tertiary proteins
What is protein synthesis-?
Protein synthesis is the process by which proteins are made in the body.
Would you find protein inside cells or cells inside protein?
Proteins are found in cells. Proteins are a macromolecule.
Are all proteins good for you?
No. Some proteins are poisons. Snake venom consists of protein. Spider venom consists of protein. Many allergens consist of proteins.
What does dna have the instructions for making?
a cell's protein
What is protein metabolism called?
protein metabolism is the metabolism of proteins
Where in the cell is protein made?
Protein is made in the ribosome.
What type of proteins does diffusion need?
proteins: suk-adic protein
