0
Western blot can be semi-quantitative. This means that you cannot determine absolute protein levels, but you can detect differences between groups.
For example, if you have 4 different samples, a western blot will be able to tell you which samples have the most of any specific cellular protein.
Quantitation is usually perfomed using "densitometry software" such as NIH ImageJ.
Wiki User
∙ 12y ago
Add your answer:
Earn +20 pts
How many people die of western blot?
I guess no one would have died by performing it. Western blot is not a dangerous technique that cause accident or induce cancer. It is just an analytic method to identify proteins.
What is western blot testing?
Western blot is an analytical method used to identify specific proteins on the sample. It is widely used in clinical labs to identify pathogens in the patient sample to conclude what disease it is. It is a powerful technique used in research labs.
When is a western blot test performed?
If the EIA test is positive then the Western Blot Test is performed
The difference between IHC and western blot?
Immunohistochemistry (IHC) is a method used to visualize protein expression in tissue samples using antibodies and staining techniques, whereas western blot is a technique that detects and quantifies specific proteins in a sample using antibodies and gel electrophoresis. IHC is used to analyze protein expression in tissue samples, while western blot is used to analyze protein expression in cell or tissue lysates.
What is western blot used for?
Western blot is used to detect any specific protein found in a sample. Normally tissue or cell lysates contain tons of different kind of proteins. By doing this analytical method one can identify the specific protein from the crude sample by antibodies.
Character of a Western blot?
Western blotting is a technique to detect specific proteins from a sample such as cell or tissue lysates. Western blot is a membrane (nitrocellulose or PVDF) on which the proteins are transferred for further analysis. Proteins on the blot are visualized by specific antibodies.
What blot test is used to confirm a seropositive ELISA test for HIV?
The western blot test
How is HIV or AIDS diagnosed?
There are several different ways to confirm HIV infection. One of the most common confirmatory testing methods is by Western Blot. There is also a confirmatory test called ELISA.
What is quantitative method?
what is quantitati
Is quantitative a research method?
No. qualitative is the research method.
What HIV test produces more accurate results than ELISA test?
There is only one test that can give the accurate results for HIV. The only test that can do this would be the Elisa test.
Is there any HIV tests?
Western Blot and ELISA
