Simple or gram? Simple uses methylene blue, gram uses crystal violet
Blue
purple
The result of simple staining of microorganisms is that they can be identified and studied under a microscope.
A Bacillus (Gram +) produces an endospore. It is called Bacillus. anthracis and causes the disease anthrax.
What are the gram reactions of clostridium and bacillus
All bacteria, including cocci, are colorless under the microscope unless you use a light filter or a stain. If you use a light filter or a stain then the color depends on which wavelength the filter is for or which type of stain you are using.
A
Methylene blue stains everything blue.
The result of simple staining of microorganisms is that they can be identified and studied under a microscope.
A Bacillus (Gram +) produces an endospore. It is called Bacillus. anthracis and causes the disease anthrax.
The malachite green penetrates the endospores, the counterstain of safranin will stain the vegetative cells. Endospores - greenish-blue Vegetative Cells - Red/Pink
Fleas carry the bacterium Yersinia pestis, formerly known as Pasteurella pestis. The plague bacillus can be stained with Giemsa stain and typically looks like a safety pin under the microscope.
There are two types of stains, the simple stain and the differential stain. A simple stain colors all objects the same while a differential stain is used to spot differences in microorganisms. A gram stain is a differential stain, which is used to tell the difference in gram negative and gram positive bacteria. A simple stain would stain all the organisms the same and this difference would not be noted. You would be able to determine their shape, whether it is a cocci or bacillus (rod), but not the type. I'm not sure why the simple stain would be preferable unless you just wanted a quick answer as to the shape of the bacteria. In some cases, a wet prep can be made of a presumptive gram positive cocci to tell the difference between bacteria or yeast. Otherwise, I would say that the gram stain is the only way to go.
B. subtilis is indeed a Gram positive rod.However, there is confusion as to whether it is an obligate aerobe or a facultative anaerobe. Although classed as an obligate aerobe, it has been shown to grow under strict anaerobic conditions.
What are the gram reactions of clostridium and bacillus
8
As the cells are transparent, the components cannot be seen clearly through a microscope. We stain the cell so that we can see the components of the cell clearly through a microscope.
When stained with iodine and observed under a compound microscope the nucleus will stain the darkest. The cheek cell is frequently employed here as they are simple to collect and tend to take the stain easily.
It's simple, because it's a stain.