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tissue culture you need cells from liver or organ
You finely chop them and you can create a primary cell line - which still has the 3d orientation of the cell. degrade it with trypsin and you have a continuous cell line. lasts 30 passages. You can immortalize it though it loses its liver key qualities. but still remains a cell non the less.
to maintain a tissue culture you need
7.4 pH
Nutrience
Glucose
Antibiotics
Serum
37 degrees
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∙ 14y ago
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