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What is the principle of allele specific oligonucleotide polymerase chain reaction?
Wiki User
∙ 14y ago
Allele specific PCR is used in the process of DNA fingerprinting where specific alleles Can be amplifies and the degree of similarity or difference between individuals can be determined.
Wiki User
∙ 14y ago
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What is primer in pcr?
A primer (oligonucleotide of a specific sequence) is required for Taq polymerase to extend the template strand by adding complementary nucleotides. The function of the primer is to anneal to the template strand at a very specific site and facilitate the initiation of strand elongation mediated by Taqploymerase.
The laboratory procedure for copying selected segments of DNA is?
The laboratory process used to copy specific segments of DNA is called the Polymerase Chain Reaction (or PCR)
What genetic locus serves as a recognition site for RNA polymerase?
The promoter region, typically located upstream of the coding sequence, serves as the recognition site for RNA polymerase. It contains specific DNA sequences that allow RNA polymerase to bind and initiate transcription.
Is an enhancer is a sequence of nucleotides that when activated by specific signal proteins aids in shielding the RNA polymerase binding site of a specific gene?
False: its An Repressor not an enhancer
Which enzyme binds to the promoter region and adds RNA nucleotides in the specific order determined by the DNA template?
RNA Polymerase
What is primer in pcr?
A primer (oligonucleotide of a specific sequence) is required for Taq polymerase to extend the template strand by adding complementary nucleotides. The function of the primer is to anneal to the template strand at a very specific site and facilitate the initiation of strand elongation mediated by Taqploymerase.
What does the medical abbreviation PCR mean?
PCR stands for "polymerase chain reaction," which is a molecular biology technique used to amplify and detect specific DNA sequences. It is commonly used in medical diagnostics and research to detect viruses, bacteria, and genetic mutations.
The laboratory procedure for copying selected segments of DNA is?
The laboratory process used to copy specific segments of DNA is called the Polymerase Chain Reaction (or PCR)
Where does RNA polymerase bind?
RNA polymerase bind specific regions of DNA called promoters. The RNA polymerase holoenzyme is guided to promoters by interactions between members of the holoenyzme and specific DNA sequences such as the TATA box.
Applicaton of archimedes' principle of specific gravity?
application of archimedes' principle of specific?
The reaction catalyzed by DNA polymerase is?
There are different types of DNA polymerase depending if it's from a eukaryotic or prokaryotic cell each performing specific tasks. Basically DNA polymerase catalyzes the formation of a polymer, a DNA strand, from many monomers, deoxyribonucleotides.
What has the author Miranda Jayne Butler written?
Miranda Jayne Butler has written: 'Identification of region-specific targets of wingless signalling in Drosophila melanogaster using oligonucleotide microarrays'
What genetic locus serves as a recognition site for RNA polymerase?
The promoter region, typically located upstream of the coding sequence, serves as the recognition site for RNA polymerase. It contains specific DNA sequences that allow RNA polymerase to bind and initiate transcription.
Differences between DNA polymerase and RNA polymerase?
A polymerase is an enzyme that catalyzes the conversion of free nucleotides into a single strand. DNA polymerase differs from RNA polymerase in two major respects: * Like all enzymes, DNA polymerase is substrate-specific. DNA polymerase cannot extend a single strand of DNA; it needs at least a short segment of double-stranded DNA at the outset. * As its name implies, DNA polymerase incorporates deoxyribonucleotides into the new strand. RNA polymerase incorporates ribonucleotides. These differences mean that DNA polymerase is active when new DNA strands are formed, as in DNA replication, and RNA polymerase is active when new RNA is formed, as in transcription. Before DNA replication can begin, the two strands must uncoil, so that each can form a template for free nucleotides to attach to. But DNA polymerase cannot get started with a single strand! In vivo(in the cell) RNA polymerase, which is active in the presence of single-stranded DNA, catalyzes the incorporation of a handful of nucleotides into a new strand. The short length of double-stranded nucleic acid that is produced enables DNA polymerase to swing into action. This still leaves a potential difficulty: the nucleotides incorporated in the presence of RNA polymerase are the wrong sort (ribonucleotides). They are subsequently replaced by DNA polymerase. In vitro (during PCR, the polymerase chain reaction) a primer, specially synthesized in a laboratory, attaches to a specific segment of single-stranded DNA, and the DNA polymerase takes over from there. The primer consists of a short length of single-stranded DNA that uniquely complements a specific DNA segment that is targeted for amplification, for example for forensic analysis.In practice, there are several different DNA polymerases and RNA polymerases in an organism.
Is an enhancer is a sequence of nucleotides that when activated by specific signal proteins aids in shielding the RNA polymerase binding site of a specific gene?
False: its An Repressor not an enhancer
What is the principle of water bath?
The water bath is used in the lab to allow a chemical reaction to occur at a specific temperature. The bath is heated to a precise temperature and the beaker or other container is placed in the bath.
What is the function of a DNA?
There are different types of DNA polymerase depending if it's from a eukaryotic or prokaryotic cell each performing specific tasks. Basically DNA polymerase catalyzes the formation of a polymer, a DNA strand, from many monomers, deoxyribonucleotides.
