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DNA fragment who are protected by a DNA binding protein are not digest. one can see the sequence recongnition
wow.....what an answer?!
DnaseI digests DNA nondiscriminantly, but at low concentrations the DNaseI nuclease cannot digest as efficiently. Only the loosely packaged chromatin is digested. this is a method used to study chromatin (DNA) packaging. Sensitivity (Hypersensitivity) to DNaseI is usually associated with actively transcribed genes, where regulatory elements are accessible to regulatory proteins. baculoboy
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What is the reagent used to test Dnase test?
There are no reagents added when a Dnase test is performed. The test is done in a methyl green medium with a pure inoculum culture. If halos form around the culture than Dnase is present.
Describe a positive DNase test?
It means that the presence of certain bacteria are there. You will need to go through the whole test to find out if this is true.
Is serratia marcescens dnase positive or negative?
Serratia genus as a whole is dnase positive.
Does DNase affect RNA?
nope.
Role of EDTA in DNA isolation?
it is chealeting agent and has great affinity with metal ions and mg- ions present in dnase as a cofactor and responsible for dnase action that degreded DNA hear edta bide with mg- ions and stop the action of dnase.
Why 1M of HCL is used in Dnase test?
what is the role of HCL in the DNase experiment
What is the reagent used to test Dnase test?
There are no reagents added when a Dnase test is performed. The test is done in a methyl green medium with a pure inoculum culture. If halos form around the culture than Dnase is present.
Why is the DNase test only incubated for 24 hrs?
For the DNase test, 24 hours is sufficient to be able to examine the culture. The test result should be recorded after this amount of time has passed, and then it must be discarded.
What is the purpose of the anti-DNase-B test?
The anti-DNase-B (ADB) test is performed to determine a previous infection of a specific type of Streptococcus, group A beta-hemolytic Streptococcus.
Does m luteus produce a positive or negative dnase test?
yes
Describe a positive DNase test?
It means that the presence of certain bacteria are there. You will need to go through the whole test to find out if this is true.
Is serratia marcescens dnase positive or negative?
Serratia genus as a whole is dnase positive.
What is an anti-DNase-B test?
detects antigens produced by group A strep, and is elevated in most patients with rheumatic fever and poststreptococcal glomerulonephritis.
Does DNase affect RNA?
nope.
Role of EDTA in DNA isolation?
it is chealeting agent and has great affinity with metal ions and mg- ions present in dnase as a cofactor and responsible for dnase action that degreded DNA hear edta bide with mg- ions and stop the action of dnase.
Principle of widal test?
same principle for latex methd
What is the principle of phosphate test?
answer it!
