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What is the procedure for Benedict's Test?
You must take the testing object, then add Benedict's solution in a test tube. Then in a boiling beaker of water, place the test tube in. Let it sit there and see if there is any color change or precipitated materials.
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Benedict reagent Principle Urinary sugars when boiled in Benedict's reagent reduce copper sulphate to a reddish cuprous oxide precipitate in hot alkaline medium, the intensity… of which is proportional to the amount of sugar present in the urine. The results are reported as I+,2+, etc. depending upon the colour and intensity of the cuprous oxide precipitate
to determine if simple sugars such as glucose and fructose are present.
Iodine tests for the presence of starch. It is brownish yellow in color if there is no starch present, and bluish black if starch is present. Benedict's solution is used to …test for the presence of a reducing sugar, changing from its usual color blue to green to brick red if reducing sugars are present. No reducing sugar solution stays blue.
Benedict's test estimates on color scale the presence of sugar .Normally used as a random pathological chemical test to determine the presence of sugar in urine in diabetes pa…tients.In presence of sugar Benedict's solution turns from blue to shades of yellow,red,brown & dark according to the percentage of sugar present in solution on heating the solution.
It acts as a control for the experiment so that you can compare the result (of benedict's test in water) with the other test (using food with sugar)
The test for a reducing sugar using benedicts solution is by, first making a colour chart, using various KNOWN concentrations of glucose situations, if available. Then use the… unknown substance and add benedicts solution and boil, a red precipitate should appear and the "redder" it is, the stronger the concentration. You can then filter off the precipitate and place the remaining liquid into a cuvette and pass through a colorimeter, on a red filter, the more light that passes through, the sronger the concentration. Compare this solution to your colour chart to help you determine the concentration of the reducing sugar. Thanks :) steph :)
to detect the presence of reducing sugars
Benedicts solution changes through a range of colours (blue, green, orange, red) according to how much reducing sugar is present in the sample. This can be used to give …a rough answer to the question "How much sugar is in the sample?", but is not accurate enough (because of the blending between one colour and the next), to be called a quantitative test.
R-CHO + 2Cu2+ + 4OH- ----------> R-COOH + Cu2O + 2H2O where R is the group attached to the aldehyde (CHO)
it indicates that there is a sugar present.
10cc test tube test tube holder alcohol lamp benedict's solution medicine dropper urine specimen
IKI test.... i think
what are the difference between these two test according to bio chemistry
Benedicts solution comprises of solution A(copper sulphate) and solution B(sodium pottasium citrate).chemically it is used as a test for aldehydes or generally carbonyl group.…It is used to measure the sugar(allose,glucose,mannose,gullose,indose,galactose,tallose)content in food.-santa
The water bath provides heat, which speeds up the reaction.
The Benedict's Test uses the Benedict's reagent, named after American chemist Stanley Rossiter Benedict. It's used to test for reducing sugars or other reducing substances…, and uses the color of the solution to determine the presence or absence of the reducing sugars.