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How does glucose react with glucose oxidase?
Since you know what Glucose Oxidase is I'm assuming you know what enzymes are. If not, an enzyme is a protein that catalyzes chemical reactions. Glucose oxidase is the enzyme built specifically for Glucose. Glucose Oxidase binds to the six-carbon sugar Glucose and aids the organism in breaking it down into metabolites.
What is the formula that use to calculate the concentration of glucose in blood by the principle of Beer-Lambert law and application of glucose oxidase?
To calculate the concentration of glucose in blood using the Beer-Lambert law principle and glucose oxidase, you would typically measure the absorbance of a glucose solution with a spectrophotometer at a specific wavelength. The formula to calculate the concentration of glucose is: Glucose concentration (mg/dL) = (Absorbance - intercept) / slope Where the slope and intercept are obtained from a calibration curve using known concentrations of glucose.
Is Pseudomonas luteola oxidase positive?
Yes, Pseudomonas luteola is oxidase-positive, meaning it contains the enzyme cytochrome C oxidase which catalyzes the oxidation of cytochrome C. This can be detected in the laboratory using an oxidase test.
If benedicts reagent turns red what substance is present?
If Benedict's reagent turns red after adding it to a solution, it indicates the presence of reducing sugars such as glucose or fructose. The red color is a result of the reduction of copper (II) ions in the reagent to copper (I) oxide by the reducing sugars.
What are the advantages of using the enzymatic method for the determination of blood glucose?
Simply put, the enzymatic method ( eg. glucose oxidase and hexokinase etc.) is the best method for blood glucose concentration because of its specificity. This yeilds maximum specificity for glucose estimation even in a comlpex mixture. Sherry-Ann Ramnarine-Phillips
How does glucose react with glucose oxidase?
Since you know what Glucose Oxidase is I'm assuming you know what enzymes are. If not, an enzyme is a protein that catalyzes chemical reactions. Glucose oxidase is the enzyme built specifically for Glucose. Glucose Oxidase binds to the six-carbon sugar Glucose and aids the organism in breaking it down into metabolites.
What is the molecular weight of glucose oxidase?
The molecular weight of glucose oxidase is approximately 160-190 kDa, depending on the specific source and form of the enzyme.
What does Benedicts reagent do?
It tests for glucose.
What is benedicts solution used to find?
Glucose
Why is GOD reagent specific to detection of glucose?
GOD (glucose oxidase) is specific to detecting glucose because it specifically catalyzes the oxidation of glucose to gluconic acid while reducing molecular oxygen to hydrogen peroxide. This reaction is unique to glucose and does not occur with other sugars, making GOD a specific enzyme for glucose detection.
Which is the enzyme which catalyses the reaction of glucose to gluconic acid?
Glucose oxidase that converts the carbonyl (aldehyde) carbon of glucose to a carboxylic acid.
How much quantity of glucose oxidase for 1 IU?
The quantity of glucose oxidase for 1 IU can vary depending on the specific enzyme preparation and assay method used. Generally, 1 IU of glucose oxidase is defined as the amount of enzyme that catalyzes the oxidation of 1 micromole of glucose per minute under specific conditions. It is typically around 1 microgram of enzyme, but it is important to refer to the manufacturer's instructions for the specific enzyme preparation being used.
What is the formula that use to calculate the concentration of glucose in blood by the principle of Beer-Lambert law and application of glucose oxidase?
To calculate the concentration of glucose in blood using the Beer-Lambert law principle and glucose oxidase, you would typically measure the absorbance of a glucose solution with a spectrophotometer at a specific wavelength. The formula to calculate the concentration of glucose is: Glucose concentration (mg/dL) = (Absorbance - intercept) / slope Where the slope and intercept are obtained from a calibration curve using known concentrations of glucose.
What color is benedicts solution when mixed with bread?
because it contains glucose
How do clinistix work?
The sticks contain enzymes (glucose oxidase and peroxidase) which react in the presense of glucose. The glucose oxidase changes glucose into gluconic acid and hydrogen peroxide and the peroxidase reacts with that hydrogen peroxide to change a pigment in the stick from neutral color to positive color.
What chemical detects glucose?
A chemical called glucose oxidase is commonly used to detect glucose. This enzyme reacts with glucose in the presence of oxygen, producing hydrogen peroxide as a byproduct. The level of hydrogen peroxide produced is then typically measured as an indicator of the glucose concentration in a sample.
Why does catalase elute in a wider range of fractions than glucose oxidase does?
Catalase elutes in a wider range of fractions than glucose oxidase due to differences in their molecular weights, structures, and interactions with the chromatography resin. Catalase is a larger protein with a more complex structure, leading to varied interactions with the resin and resulting in a broader elution profile. Glucose oxidase, being smaller and less complex, interacts more specifically with the resin, leading to a narrower elution range. Additionally, differences in the hydrophobicity, charge, and binding affinity of the two enzymes also contribute to their distinct elution profiles.
