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Why does catalase elute in a wider range of fractions than glucose oxidase does?
Catalase elutes in a wider range of fractions than glucose oxidase due to differences in their molecular weights, structures, and interactions with the chromatography resin. Catalase is a larger protein with a more complex structure, leading to varied interactions with the resin and resulting in a broader elution profile. Glucose oxidase, being smaller and less complex, interacts more specifically with the resin, leading to a narrower elution range. Additionally, differences in the hydrophobicity, charge, and binding affinity of the two enzymes also contribute to their distinct elution profiles.
Why is GOD reagent specific to detection of glucose?
GOD (glucose oxidase) is specific to detecting glucose because it specifically catalyzes the oxidation of glucose to gluconic acid while reducing molecular oxygen to hydrogen peroxide. This reaction is unique to glucose and does not occur with other sugars, making GOD a specific enzyme for glucose detection.
Which is the enzyme which catalyses the reaction of glucose to gluconic acid?
Glucose oxidase that converts the carbonyl (aldehyde) carbon of glucose to a carboxylic acid.
What is the molecular weight of Dextrin?
The average molecular weight of dextrin can vary depending on the degree of polymerization and branching. Typically, dextrin has a molecular weight ranging from 500 to 5,000 g/mol.
Which sugar or sugars are substrates for glucose oxidase?
Glucose oxidase primarily acts on glucose as its substrate. It catalyzes the oxidation of glucose to gluconic acid while producing hydrogen peroxide. While glucose is the main substrate, some forms of the enzyme can also act on other sugars, such as D-fructose, but with significantly lower efficiency.
Glucose molecular weight in Dalton unit?
The molecular weight of glucose is approximately 180.16 daltons.
Why does catalase elute in a wider range of fractions than glucose oxidase does?
Catalase elutes in a wider range of fractions than glucose oxidase due to differences in their molecular weights, structures, and interactions with the chromatography resin. Catalase is a larger protein with a more complex structure, leading to varied interactions with the resin and resulting in a broader elution profile. Glucose oxidase, being smaller and less complex, interacts more specifically with the resin, leading to a narrower elution range. Additionally, differences in the hydrophobicity, charge, and binding affinity of the two enzymes also contribute to their distinct elution profiles.
How does glucose react with glucose oxidase?
Since you know what Glucose Oxidase is I'm assuming you know what enzymes are. If not, an enzyme is a protein that catalyzes chemical reactions. Glucose oxidase is the enzyme built specifically for Glucose. Glucose Oxidase binds to the six-carbon sugar Glucose and aids the organism in breaking it down into metabolites.
What kind of macromolecule is insulin?
Glucose is grouped as a Carbohydrate.
Why divide glucose mgs by 180 for millimoles?
Because the formula for calculating moles is dividing grams by the molecular weight. The molecular weight for glucose is 180. grams to moles is also the same as milligrams to millimoles.
What molecule is larger glucose or albumin?
Glucose molecules are larger than water molecules.
Equivalent weight of glucose?
The equivalent weight of glucose is 180 g/mol. This is calculated by dividing the molecular weight of glucose (180 g/mol) by the number of equivalents of reactive atoms in the compound, which in this case is 1.
The molecular weight of free glucose is 180.2 while that of glucose in glycogen is 160 explain this anomaly with structural diagrams?
Put the following glucose solutions in order of least concentrated (A) to most concentrated (E) (note: mw of glucose is 180)10 grams glucose in 75 millilitres of water600 mM glucose5 mg in 1 mL water7.5 mM glucose in 10 mM NaCl10 % (w/v) glucose
Why is GOD reagent specific to detection of glucose?
GOD (glucose oxidase) is specific to detecting glucose because it specifically catalyzes the oxidation of glucose to gluconic acid while reducing molecular oxygen to hydrogen peroxide. This reaction is unique to glucose and does not occur with other sugars, making GOD a specific enzyme for glucose detection.
If the atomic weight of carbon is twelve and the atomic weight of oxygen is sixteen the molecular weight of glucose is?
Glucose: C6 H12 O6 Carbon: 6 times 12 = 72 Hydrogen: 12 times 1 (actually 1.008) = 12 Oxygen: 6 times 16 = 96 All together that makes the molecular weight of Glucose is 180
Which is the enzyme which catalyses the reaction of glucose to gluconic acid?
Glucose oxidase that converts the carbonyl (aldehyde) carbon of glucose to a carboxylic acid.
What do the two molecules ( fructose and glucose ) have in common?
Fructose and Glucose are isomers of each other. That means that one part of the molecule is in a different location on the other molecule. Fructose and glucose have the same molecular formula and molecular weight.
