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Since you know what Glucose Oxidase is I'm assuming you know what enzymes are. If not, an enzyme is a protein that catalyzes chemical reactions.
Glucose oxidase is the enzyme built specifically for Glucose. Glucose Oxidase binds to the six-carbon sugar Glucose and aids the organism in breaking it down into metabolites.
What else can I help you with?
Why is the Glucose Oxidase test a more specific test for urinary glucose that Benedict's test?
The Glucose Oxidase test specifically measures the presence of glucose by detecting its oxidation reaction with glucose oxidase enzyme. This enzyme only reacts with glucose, making the test highly specific for glucose detection. On the other hand, Benedict's test, which relies on the reduction of copper ions, can give false positive results with other reducing sugars present in the urine, leading to lower specificity for glucose.
Is Pseudomonas luteola oxidase positive?
Yes, Pseudomonas luteola is oxidase-positive, meaning it contains the enzyme cytochrome C oxidase which catalyzes the oxidation of cytochrome C. This can be detected in the laboratory using an oxidase test.
What is the formula that use to calculate the concentration of glucose in blood by the principle of Beer-Lambert law and application of glucose oxidase?
To calculate the concentration of glucose in blood using the Beer-Lambert law principle and glucose oxidase, you would typically measure the absorbance of a glucose solution with a spectrophotometer at a specific wavelength. The formula to calculate the concentration of glucose is: Glucose concentration (mg/dL) = (Absorbance - intercept) / slope Where the slope and intercept are obtained from a calibration curve using known concentrations of glucose.
What happens when glucose and starch react together?
Glucose and starch do not react together chemically. However, enzymes such as amylase can break down starch into glucose through a process called hydrolysis. This allows the glucose to be used for energy production in the body.
How can you easily distinguish enterics and pseudomonads?
Enterics are typically Gram-negative bacteria that ferment glucose, produce acid, and are often found in the intestines of animals. Pseudomonads are also Gram-negative bacteria, but they do not ferment glucose. Additionally, pseudomonads are known for their ability to produce pyocyanin pigment and grow in diverse environments, such as soil and water.
How do clinistix work?
The sticks contain enzymes (glucose oxidase and peroxidase) which react in the presense of glucose. The glucose oxidase changes glucose into gluconic acid and hydrogen peroxide and the peroxidase reacts with that hydrogen peroxide to change a pigment in the stick from neutral color to positive color.
What is the molecular weight of glucose oxidase?
The molecular weight of glucose oxidase is approximately 160-190 kDa, depending on the specific source and form of the enzyme.
Which is the enzyme which catalyses the reaction of glucose to gluconic acid?
Glucose oxidase that converts the carbonyl (aldehyde) carbon of glucose to a carboxylic acid.
Which sugar or sugars are substrates for glucose oxidase?
Glucose oxidase primarily acts on glucose as its substrate. It catalyzes the oxidation of glucose to gluconic acid while producing hydrogen peroxide. While glucose is the main substrate, some forms of the enzyme can also act on other sugars, such as D-fructose, but with significantly lower efficiency.
Why is the Glucose Oxidase test a more specific test for urinary glucose that Benedict's test?
The Glucose Oxidase test specifically measures the presence of glucose by detecting its oxidation reaction with glucose oxidase enzyme. This enzyme only reacts with glucose, making the test highly specific for glucose detection. On the other hand, Benedict's test, which relies on the reduction of copper ions, can give false positive results with other reducing sugars present in the urine, leading to lower specificity for glucose.
What of these substances is an enzyme A. triglyceride B. glucose C. oxidase D. omega-3 fatty acid E. nucleotide?
C. Oxidase is an enzyme.
Why does catalase elute in a wider range of fractions than glucose oxidase does?
Catalase elutes in a wider range of fractions than glucose oxidase due to differences in their molecular weights, structures, and interactions with the chromatography resin. Catalase is a larger protein with a more complex structure, leading to varied interactions with the resin and resulting in a broader elution profile. Glucose oxidase, being smaller and less complex, interacts more specifically with the resin, leading to a narrower elution range. Additionally, differences in the hydrophobicity, charge, and binding affinity of the two enzymes also contribute to their distinct elution profiles.
Is Pseudomonas luteola oxidase positive?
Yes, Pseudomonas luteola is oxidase-positive, meaning it contains the enzyme cytochrome C oxidase which catalyzes the oxidation of cytochrome C. This can be detected in the laboratory using an oxidase test.
What is glucose oxidase peroxidase aminophenazone phenol?
Its a reagent used to determine the glucose content of a sample. its based on 2 coupled enzyme reactions with a colorimetric end-point: D-glucose + O2 + H2O ----- glucose oxidase ------> H2O2 + gluconate aminophenazone + phenol + H2O2 -----peroxidase----> a red dye + H2O (NOTE: H2O2 product of 1st reaction acts as substrate for 2nd) this red coloured sample can then be put into a spectrophotometer and an absorbency reading can be taken, this reading can be compared to a calibration curve and the content of glucose can be ascertained.
When glucose is burned which gas in the air does the glucose react with?
It is oxygen.
How much quantity of glucose oxidase for 1 IU?
The quantity of glucose oxidase for 1 IU can vary depending on the specific enzyme preparation and assay method used. Generally, 1 IU of glucose oxidase is defined as the amount of enzyme that catalyzes the oxidation of 1 micromole of glucose per minute under specific conditions. It is typically around 1 microgram of enzyme, but it is important to refer to the manufacturer's instructions for the specific enzyme preparation being used.
What is the formula that use to calculate the concentration of glucose in blood by the principle of Beer-Lambert law and application of glucose oxidase?
To calculate the concentration of glucose in blood using the Beer-Lambert law principle and glucose oxidase, you would typically measure the absorbance of a glucose solution with a spectrophotometer at a specific wavelength. The formula to calculate the concentration of glucose is: Glucose concentration (mg/dL) = (Absorbance - intercept) / slope Where the slope and intercept are obtained from a calibration curve using known concentrations of glucose.
