The cell walls of some bacteria eg mycobacteria contain mycolic acids giving the cell walls a high lipid content. To view these cells in samples staining requires higher concentrations of the dye solution and/or a heating period. However, once a stain is introduced into the cell wall, removing it with a decolorising is even more difficult. The expression "acid fast" is derived from the observation that even with the addition of hydrochloric acid to the alcohol decoloriser, some of the stained cells retain the primary stain (carbolfuchsin).
Acid fast refers to microorganisms whose cell wall has a high lipid content of mycolic acids and long chain fatty acids. The wall is said to be thicker.
to detect bacterial stracture
to detect bacterial stracture
Endospore staining is a differential stain used to detect the presence and location of spores in bacterial cells.
Staining is a chemical process.
staining affinity refers to the molecular attraction of a specific stain for a particular type of cell component.
A basic dye used in gram staining is crystal violet.
affix the cells to the slide and to kill bacteria
simple
In Gram staining procedure during bacterial staining , iodine forms a complex with crystal violet stain which stains Gram positive bacteria blue to violet .
Capsules may be revealed by methods such as Maneval's method. This method utilizes negative staining, where the background is stained revealing an unstained structure: the bacterial capsule.
The component of madre de cacao leaves is tannins. Tannins is also found in tea leaves and known for its staining properties.
Adding methylene blue to a slide will stain animal cells and make the nuclei more visible.