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affix the cells to the slide and to kill bacteria
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During the performance of the simple staining procedure you failed to heat fix your EColi smear preparation Upon microscopic examination how would you expect this slide to differ from correct slide?
The bacterial smear will wash away during the staining procedure. This is avoided by heat fixation, during which the bacterial proteins are coagulated and fixed to the glass surface.
Why is the slide not heat fixed before negative staining?
First and foremost, the purpose of heat fixing is to drive stain into the bacterial cells, which in this case, you are staining the background, so there is not a need for heat fixing. Next, the process of heat fixing will shrink the cell by a little. This sorts of support the first reason as since there isn't the need to heat fix, then don't. By not heat-fixing, we actually see a more accurate morphology, arrangement and size of thr bacterial cell. Hope that my answers helps 😊
What are the advantages of the simple stain technique over the negative stain technique?
Negative staining has a dark contrasted background and the bacteria is white. Simple staining has a white background and bacteria is the color depended on your stain color.Negative staining when prepared is NOT heat fixed but simple staining when prepared is heat fixed. Heat fixed means when preparing slide with bacteria on it, it is passed over some type of flame, like a Bunsen burner flame, three times or four times.
What would you expect from a gram stain done on a slide that was heated too hot during the heat-fixed smear?
A Gram stain refers to a staining technique for the identification of bacteria. A Gram stain done on a slide that was heated too hot during the heat-fixed smear will destroy the cell wall of the bacteria.
What is the purpose of emulsifying the sample serum in staining procedure?
To promote the cells adhering to the glass slide since they can't be heat fixed. When preparing a capsule stain you have to aseptically add organisms and emulsify with a loop.
During the performance of the simple staining procedure you failed to heat fix your EColi smear preparation Upon microscopic examination how would you expect this slide to differ from correct slide?
The bacterial smear will wash away during the staining procedure. This is avoided by heat fixation, during which the bacterial proteins are coagulated and fixed to the glass surface.
Why is the slide not heat fixed before negative staining?
First and foremost, the purpose of heat fixing is to drive stain into the bacterial cells, which in this case, you are staining the background, so there is not a need for heat fixing. Next, the process of heat fixing will shrink the cell by a little. This sorts of support the first reason as since there isn't the need to heat fix, then don't. By not heat-fixing, we actually see a more accurate morphology, arrangement and size of thr bacterial cell. Hope that my answers helps 😊
Can cells be stained with dye?
Live staining is possible as is the preparation of fixed tissue.
What are the advantages of the simple stain technique over the negative stain technique?
Negative staining has a dark contrasted background and the bacteria is white. Simple staining has a white background and bacteria is the color depended on your stain color.Negative staining when prepared is NOT heat fixed but simple staining when prepared is heat fixed. Heat fixed means when preparing slide with bacteria on it, it is passed over some type of flame, like a Bunsen burner flame, three times or four times.
What is a primary stain?
If your talking about Acid Fast staining (aka Ziehl-Neelsen staining), after the addition of the primary stain (carbol fuchsin) heat is applied in order to facilitate proper staining. Bacteria such as Mycobacterium contain large amounts of lipid substances within their cell wall called mycolic acids. These acids resist staining by ordinary methods such as gram staining (where heat is not applied after primary staining). On application of heat, the stain carbol fuschin penetrates the cell wall and stains the cells pink. Following the secondary staining (methylene blue) the acid fast positive cells appear pink while others are stained blue. Endospore staining is yet another staining technique where heat is applied after primary staining (malachite green). In this case the spores are impermeable to stains, hence heating favours proper permeation of stain. Endospores appear green while vegetative cells appear red (secondary stain saffranine). Not all staining procedures involve applying heat after primary staining. However, heat is applied before even beginning the staining procedure. This is called heat fixing, where the cells are fixed so that they are not washed away during the subsequent washing steps.
Why steam heating is done in acid fast staining and not heat fixing?
Actually, both methods are used during the staining procedure (steam & heat fix). Initially, the organism is heat fixed to the slide to prevent the organism from being washed off during subsequent steps. Later in the procedure, the slide with the heat fixed organism is steamed to make the cell wall a little more penetrable - allowing the stain to enter the cell wall.
What is nitrogen changed into before being used by living things?
lightning & bacterial action (nitrogen fixers).
Nitrogen in the atmosphere must be fixed before it can be used by plants. Atmospheric nitrogen is fixed by?
bacteria and lighntning
What is gram staining?
Gram Staining is a way to separate one large groups of bacteria into two. Crystal violet is used to dye the cells, those that retain the color are grouped as Gram-positive, and those that do not retain the color are grouped as Gram-negative. Many of the Gram-negative bacteria are pathogenic, making this process useful for detecting infections. A link is provided to permit a quick trip to the Wikipedia article on this topic.
What is the meaning of fixed overhead total variances in an absorption costing system?
Fixed overhead variance means actual fixed overhead cost was more than it was actually budgeted before start of operations.
Does a comma go before a connective or after?
Before eg. The television has broken, therefore I will get it fixed.
What is the principle of crystal violet staining in monolayer culture?
Crystal Violet is a dye that accumulates in the cell nucleus. The fixed dye, measured photometrically after solubilization, correlates with the nuclear DNA content and thus with cell number.
