Annihilate all other living things and sunder all organic complexes.
In a lab setting this can be well enough achieved by wiping down counter tops with alcohol, or mid-procedure you can place metal instruments into a flame until they are red hot.
If an experiment could be interrupted by organisms that survive these methods of sterilization and those organisms are likely to be present then further steps should be taken to remove them but such cases would be rare and not worth worrying about in, say, college courses.
Low temp is not a good option for sterlization but yes we can do that with etch thying.
Steam sterilization, or using an autoclave to heat objects, is a good way to sterilize tools. Ionizing radiation can also be used to sterilize things.
streptomycin
dettol
washing up liquid
tcp
milton
iodine
Autoclave
dry heat sterilization
When a liquid is heat labile or can be destroyed by heat then this method is used.
Heat is the physical method used to control microbial growth via the mechanism of protein denaturation. By exposing microbes to high temperatures, the heat disrupts and denatures the proteins in their cells, leading to their inactivation and ultimately killing them. This method is commonly used in processes such as pasteurization and autoclaving to sterilize equipment and destroy harmful microorganisms.
If we limit our discussion to the enveloped viruses HIV, HCV and HBV, then lyophilization is a sterilization method as explained in my recent article [Cell Tissue Bank (2011) 12:99-104]. Desiccation of the lipid envelops inactivates these viruses.
Moist heat, or heat in the form of saturated steam under pressure, is widely used as a sterilization method because it allows for temperatures higher than what can be reached by boiling. It disinfects and sterilizes the life of microorganisms by coagulating its protein.
Oral contraceptives (or "the pill") Sterilization
sterilization
The only 100% effective means is abstinence- not to have sex. Surgical sterilization, such as removal of the uterus is also highly effective. All common means of birth control such as condoms, birth control pills, IUDs, withdrawal, rhythm method CAN fail in some percentage of cases.
England
Autoclaving is a method of sterilizing equipment by the use of pressurized high temperature steam. It may be used to sterilize surgical equipment for reuse or for sterilizing bottles prior to filling them with beer, soda pop, or other beverages.
Advantages: 1. It is faster method of sterilization then dry heat method. 2. Additional parameter "pressure" is involved 3. We can easily sterilize the media solution. 4. It faster method of sterilization. Disadvantages: 1. Glass ware might get broken down. 2. Mishandling may lead to serious accident or explosion.
When a liquid is heat labile or can be destroyed by heat then this method is used.
dry heat sterilization
Sterilization can be accomplished by heat (as in the autoclave), chemicals (as with glutaraldehyde) or radiation. Your average outpatient office uses heat or chemical sterilization.
Condoms, IUDs, birth control pills, depot birth control medications (e.g. monthly, or quarterly), morning-after pill methods, contraceptive sponge, cervical cap, sterilization ... and the least effective method of contraception - the "rhythm method".
Steam sterilization uses much less energy and time than dry heat methods. In order to kill spores a dry heat sterilizer needs to reach and maintain 170°C for one hour. An autoclave only needs to reach 121°C for 15 minutes.
Heat is the physical method used to control microbial growth via the mechanism of protein denaturation. By exposing microbes to high temperatures, the heat disrupts and denatures the proteins in their cells, leading to their inactivation and ultimately killing them. This method is commonly used in processes such as pasteurization and autoclaving to sterilize equipment and destroy harmful microorganisms.