If we limit our discussion to the enveloped viruses HIV, HCV and HBV, then lyophilization is a sterilization method as explained in my recent article [Cell Tissue Bank (2011) 12:99-104]. Desiccation of the lipid envelops inactivates these viruses.
dry heat sterilization
Conductivity. Moisture conducts the heat better than a dry system.
Dry-heat sterilization is generally a less complicated process than steam sterilization; it is, however, relatively slow and requires higher temperatures and/or longer exposure times. This is because of the fact that microbial lethality is lower with dry heat than that for steam at the same temperature.
we have 4 types for dry heat steralization 1.red heat eg. heating of platinum loop,points of forceps 2.flaming eg. used for needles,mouth of culture tubes 3.hot air oven temp. of 160 c fpr 1 hr is employed and its the best method for stterelization dry glasswares,forceps,scalpels,powders,fat oils, greases. 4.infra red radiation the source employed is an electrically heat element a temp. of 180 c can be attained
It is easier to use
dry heat sterilization
Advantages: 1. It is faster method of sterilization then dry heat method. 2. Additional parameter "pressure" is involved 3. We can easily sterilize the media solution. 4. It faster method of sterilization. Disadvantages: 1. Glass ware might get broken down. 2. Mishandling may lead to serious accident or explosion.
Conductivity. Moisture conducts the heat better than a dry system.
Pasteurization
Steam sterilization uses much less energy and time than dry heat methods. In order to kill spores a dry heat sterilizer needs to reach and maintain 170°C for one hour. An autoclave only needs to reach 121°C for 15 minutes.
Dry-heat sterilization is generally a less complicated process than steam sterilization; it is, however, relatively slow and requires higher temperatures and/or longer exposure times. This is because of the fact that microbial lethality is lower with dry heat than that for steam at the same temperature.
A hot air oven is usually used for sterilizing items. They are very common in places like tattoo parlors, for example.
Dry heat = 1-2hrs @ 160-170 degrees Celsius Moist heat = 15min. @ 121 degrees Celsius
Mechanism of killing by dry heatDry heat kills the organisms by destructive oxidation of essential cell constituentsKilling of the most resistant spores by dry heat requires a temperature of about 160 °C for 60 minutesDry heat is employed for glassware; syringes, metal instruments and paper wrapped goods, which are not spoiled by high temperatures.It is also used for anhydrous fats, oils and powders that are impermeable to moisture.Mechanism of killing by moist heatMoist heat kills the organisms by coagulating and denaturing their enzymes and structural protein.Sterilization by moist heat of the most resistant spores generally requires 121 °C for 15-30 minutes.Moist heat is used for the sterilization of culture media, and all other materials through which steam can penetrateMoist heat is more effective than dry heatSterilization can be done at lower temperatures in a given time at a shorter duration at the same temperature.upon heating wet proteins release free -sh groups and form smaller peptide chains which in turn form new complexes different from the orignal protein molecules
Sterilization by various means such as steam sterilization (autoclave), dry heat (hot air oven), radiation (ionising & non-ionising),chemical methods etc. kills microorganisms.
what method did earliest people use to cook food first? dry heat or moist heat
Convection.