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Dry-heat sterilization is generally a less complicated process than steam sterilization; it is, however, relatively slow and requires higher temperatures and/or longer exposure times. This is because of the fact that microbial lethality is lower with dry heat than that for steam at the same temperature.

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16y ago
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13y ago

Sterilization with steam under pressure. mainly at 121 degrees C, 15 pounds of pressure and for 20 mins.

Pressure should be 1.2 Kg/cm

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Q: What is the definition of moist heat sterilization?
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Which is hotter moist heat or dry heat?

Conductivity. Moisture conducts the heat better than a dry system.


Methods involved in moist heat sterilization below hundred degree celsius?

WELL! moist heat is used by heating with steam and the steam can not be formed below 100oC so it is not possible to kill microorganism by moist heat below hundred degree


What is the relationship of time to temperatures in heat sterilization?

Dry heat = 1-2hrs @ 160-170 degrees Celsius Moist heat = 15min. @ 121 degrees Celsius


Why Moist heat sterilization is more effective than Dry heat sterilization?

Mechanism of killing by dry heatDry heat kills the organisms by destructive oxidation of essential cell constituentsKilling of the most resistant spores by dry heat requires a temperature of about 160 °C for 60 minutesDry heat is employed for glassware; syringes, metal instruments and paper wrapped goods, which are not spoiled by high temperatures.It is also used for anhydrous fats, oils and powders that are impermeable to moisture.Mechanism of killing by moist heatMoist heat kills the organisms by coagulating and denaturing their enzymes and structural protein.Sterilization by moist heat of the most resistant spores generally requires 121 °C for 15-30 minutes.Moist heat is used for the sterilization of culture media, and all other materials through which steam can penetrateMoist heat is more effective than dry heatSterilization can be done at lower temperatures in a given time at a shorter duration at the same temperature.upon heating wet proteins release free -sh groups and form smaller peptide chains which in turn form new complexes different from the orignal protein molecules


What is moist sterilization?

it is steam sterlization in which temp & pressure both works


Under what conditions is sterilization by filtration preferred over sterilization by heat?

When a liquid is heat labile or can be destroyed by heat then this method is used.


What are the three instrument classifications that are used to determine the method of sterilization?

Sterilization can be accomplished by heat (as in the autoclave), chemicals (as with glutaraldehyde) or radiation. Your average outpatient office uses heat or chemical sterilization.


What is the effect of moist heat on sugar?

What effect of moist heat sugar


What are the application and relative advantages and disadvantages of dry heat and moist heat sterilization?

Advantages: 1. It is faster method of sterilization then dry heat method. 2. Additional parameter "pressure" is involved 3. We can easily sterilize the media solution. 4. It faster method of sterilization. Disadvantages: 1. Glass ware might get broken down. 2. Mishandling may lead to serious accident or explosion.


What substances Destroy the life of microorganism by coagulating its protein?

Moist heat, or heat in the form of saturated steam under pressure, is widely used as a sterilization method because it allows for temperatures higher than what can be reached by boiling. It disinfects and sterilizes the life of microorganisms by coagulating its protein.


What is the heat process which reduces pathogens to a safe level?

sterilization


If a person wants to make a room free from microbes and their spores what sterilization method would you advice him to use?

dry heat sterilization