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Why colonies of same color are different in sizes in pour plate method?
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What is the morphology of colonies of E. coli grown on MCA medium by streak plate method?
G+ and tan
What are 3 methods used to isolate microorganisms from a mixed culture?
It is necessary to make the colonies well-isolated from each other so that each appears distinct, large and shows characteristic growth forms.Most bacteria, many other microfungi, and unicellular microalgae, may be most commonly obtained by plating methods such as streak plate method, pour plate method and spread plate method.
Who was the first to developed the culture plate method to identify pathogens?
Rober Kock developed the culture plate method to identify pathogens.
What is considered a high plate count?
Usually hundreds of colonies (>300) is considered as a high plate count.
What are the surface colonies on a pour plate larger than those within the medium?
The surface colonies on a pour plate larger than those within the medium especially aerobic bacteria within the medium would be a restriction of growth. The restriction of growth would be due to the lack of oxygen.
What does the streak plate method have over the pour plate method?
It is more likely to give individual colonies regardless of the concentration of the original source. With pour plates, you might have to use several plates with different dilutions of inoculum to get individual colonies.
What advantages does the streak-plate method have over the pour plate method?
The streak plate method makes it easier for colonies of bacteria to grow. It also generally leads to individual colonies that look like small dots, rather then simply a mat of bacterial growth.
What advantages does the pour plate method have over streak plate method?
The streak plate method makes it easier for colonies of bacteria to grow. It also generally leads to individual colonies that look like small dots, rather then simply a mat of bacterial growth.
Which method often results in colonies developing down throughout the agar and some colonies on the surface?
pour plate
The technique in which bacteria from all of the colonies of one plate are simultaneously trasnferred to the same position on another plate is called?
Replica plating method.
What is the morphology of colonies of E. coli grown on MCA medium by streak plate method?
G+ and tan
What color are the colonies of E Coli when growing on a nutrient agar plate?
whitw to whitish gray
How do you measure bacteria in a petri dish?
When bacteria is grown in an Agar plate, one quantitative method to measure growth is using a counting chamber. Another method is using viable plate counts.
How can you tell if a mixed culture contains more than one type of bacteria?
You will see different color colonies, irregular ones, or round and even ones on a streak plate.
What advantage does the streak plate method have over the pour plate method?
A disadvantage of the streak plate technique could be colony isolation problems. If the streaking technique is not done properly or if there is too much of an organism present on the inoculating loop then the cells can cluster and form what looks like one colony but it can actually be a couple colonies (made from a couple cells). This can cause an inaccurate colony forming unit count.
Why Singapore have different plate number color?
7
Why did the colonies on the MAC plate turn pink?
The colonies that grew on MAC plate took up lactose from the medium for their metabolism and released an end product that caused the pH indicator of the medium (neutral red) to turn pink. Hence the colonies appears pink in color.
