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What is the morphology of colonies of E. coli grown on MCA medium by streak plate method?
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∙ 10y ago
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What is the main advantage of T-streak?
The main advantage of T- streak method: 1. To get a very good isolated colonies.2. To obtain pure culture from mixed culture.
Why must have 4 part in streaking technique?
so that you can get isolated colonies in the last streak . . . As you streak contineously you inoculum quantity decreases . . there by when you reach the end of last streak you get separate and isolated colonies . .
Why would bacterial colonies found in the first section of a streak plate but not on sections two and three?
why could bacterial colonies found in the first section of a streak plate but not on sections two and three
What is the purpose of the streak for isolation?
By using streak plate technique to spread a clinical sample out on the surface of a growth medium individual types of bacteria can be isolated
What are the steps in properly streaking a plate?
When we have to isolate a specific microbial species from their mix culture or to grow any microbe on solid surface for their further studies then they can be grow on a culture medium containing a gel like substance known as agar which produce disticnt microbial colonies when inoculate in a petri dish containing the growth medium. The way by which the inoculation of microbial sample done is called a streak plate method in which the microbial sample is streak with the help of inoculating loop on the agar plate firmly, in the way so that the cell can be isolated. There are two more method to incoulate the microbial sample that are: pour plate and spread plate techniques.
What advantages does the streak-plate method have over the pour plate method?
The streak plate method makes it easier for colonies of bacteria to grow. It also generally leads to individual colonies that look like small dots, rather then simply a mat of bacterial growth.
What advantages does the pour plate method have over streak plate method?
The streak plate method makes it easier for colonies of bacteria to grow. It also generally leads to individual colonies that look like small dots, rather then simply a mat of bacterial growth.
What is the main advantage of T-streak?
The main advantage of T- streak method: 1. To get a very good isolated colonies.2. To obtain pure culture from mixed culture.
What is the main advantage of T streak?
The main advantage of T- streak method: 1. To get a very good isolated colonies.2. To obtain pure culture from mixed culture.
What does the streak plate method have over the pour plate method?
It is more likely to give individual colonies regardless of the concentration of the original source. With pour plates, you might have to use several plates with different dilutions of inoculum to get individual colonies.
Why use a streak plate to grow a bacterium?
You do a streak plate in order to get isolated colonies. If you inoculate into a slant, you have less surface area to work and less chance of getting isolated colonies. In broth, you'll definitely get growth but you won't know WHAT is growing. You go back into each quadrant (a little) with your loop in order to "dilute" the bacteria and get colonies. Quadrant 1 is pretty think (like a smear on the plate) but by the time you get to Quadrants 3 and 4, you should see more defined colonies and not just a film of bacteria.
Where should colonies appear in the case of streak plates?
When using streak plates, the colonies should appear along the streak lines. This is where the bacteria have been introduced and is the first place they will grow.
Why must have 4 part in streaking technique?
so that you can get isolated colonies in the last streak . . . As you streak contineously you inoculum quantity decreases . . there by when you reach the end of last streak you get separate and isolated colonies . .
What will happen if the streak plate were incubated a month?
The streak lines would show a lot of colonies.
What advantage does the streak plate method have over the pour plate method?
A disadvantage of the streak plate technique could be colony isolation problems. If the streaking technique is not done properly or if there is too much of an organism present on the inoculating loop then the cells can cluster and form what looks like one colony but it can actually be a couple colonies (made from a couple cells). This can cause an inaccurate colony forming unit count.
How you differentiate between Transformed bacterial colonies and satellite colonies?
Re-streak the center of the 'star' colony (transformed surrounded by satellites) on a plate contains the antibiotic, typically ampicillin. The colonies in the tertiary streak will most likely be the transformants. If you want to be quite sure, pick a single colony from the tertiary streak and re-streak again on a plate containing the antibiotic.
List two methods for obtaining isolated colonies.?
Use a "streak plate" or a "spread plate". Mediums that allow some organisms to grow and not others can also be used. These types of mediums are called Selective medium.
