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Why would bacterial colonies found in the first section of a streak plate but not on sections two and three?
Wiki User
∙ 13y ago
why could bacterial colonies found in the first section of a streak plate but not on sections two and three
Wiki User
∙ 13y ago
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Why must have 4 part in streaking technique?
so that you can get isolated colonies in the last streak . . . As you streak contineously you inoculum quantity decreases . . there by when you reach the end of last streak you get separate and isolated colonies . .
What is the function of pound Agar?
LB Broth allows you to create a suspension of bacterial cells from your original colony growing on an agar plate. It provides the necessary nutrients and environment for optimal replication. In suspension, bacterial cells can be plated onto more agar plates, in order to create a streak to cultivate colonies from a single cell, or used to in purification reaction in order to extract the transformed plasmid.
What is the main advantage of T-streak?
The main advantage of T- streak method: 1. To get a very good isolated colonies.2. To obtain pure culture from mixed culture.
What is the morphology of colonies of E. coli grown on MCA medium by streak plate method?
G+ and tan
What is the streak of conglomerate?
what is the streak of sedimentary rocks
How you differentiate between Transformed bacterial colonies and satellite colonies?
Re-streak the center of the 'star' colony (transformed surrounded by satellites) on a plate contains the antibiotic, typically ampicillin. The colonies in the tertiary streak will most likely be the transformants. If you want to be quite sure, pick a single colony from the tertiary streak and re-streak again on a plate containing the antibiotic.
What advantages does the streak-plate method have over the pour plate method?
The streak plate method makes it easier for colonies of bacteria to grow. It also generally leads to individual colonies that look like small dots, rather then simply a mat of bacterial growth.
What advantages does the pour plate method have over streak plate method?
The streak plate method makes it easier for colonies of bacteria to grow. It also generally leads to individual colonies that look like small dots, rather then simply a mat of bacterial growth.
Where should colonies appear in the case of streak plates?
When using streak plates, the colonies should appear along the streak lines. This is where the bacteria have been introduced and is the first place they will grow.
Why must have 4 part in streaking technique?
so that you can get isolated colonies in the last streak . . . As you streak contineously you inoculum quantity decreases . . there by when you reach the end of last streak you get separate and isolated colonies . .
What will happen if the streak plate were incubated a month?
The streak lines would show a lot of colonies.
The purpose or objective of streak plating is what?
to under stand the principle of bacterial isolation
If an inoculated plate had colonies between the streak lines what would you conclude?
It depends, if there is no growth or colony appearance on streak line and only it shows growth in b/w the streak line then it is certainly a contamination and if there are colonies on streak line and not ressemble with the streak culture then also it is a contamination but there can be a chance that colony appears due to some fault in streaking procedure and the inoculum drops between the streak line so it depends.
If you were given a tube with 3 different bacterial species in it what is the first thing you would do to separate the three?
The best thing to do is get a nutrient agar plate and spread the bacteria using a streak-plate method of isolation to grow the different colonies individually. (And you could do a second streak-plate from each type of colony you see just to make sure that your colonies each contain only one type of bacteria.) From there you can identify and grow each pure culture. (Also, you could use selective medias.)
What is the function of pound Agar?
LB Broth allows you to create a suspension of bacterial cells from your original colony growing on an agar plate. It provides the necessary nutrients and environment for optimal replication. In suspension, bacterial cells can be plated onto more agar plates, in order to create a streak to cultivate colonies from a single cell, or used to in purification reaction in order to extract the transformed plasmid.
Why is a needle used to isolate individual colonies from a spread plate or streak plate?
Between each set of streaks you sterilize the inoculating loop in the Bunsen flame. At the beginning of the next streak you overlap with the end of the one before. The effect of the technique is to "dilute" the bacteria by gradually spreading then over greater distances. Eventually, a point is reached where single bacteria are spaced sufficiently far apart for single colonies to grow without infringing their neighbors.
Why do you cross over back the 2nd streak section back into 1st section and from the 3rd section back across the 2nd section?
Bcs to get less dense colony
