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Bacteria is stained to identify the organism and its structure. Staining also can help in identification of a species. Bacteria are translucent in appearance therefore a stain is applied to allow them to become visible.
Wiki User
∙ 14y ago
Wiki User
∙ 15y agoThe purpose of staining is:
1) - stains make microbes stand out against the background.
2) - helps investigators group major categories
3) - helps to distinguish parts of a cell.
Wiki User
∙ 14y agowe stain bacteria to allow them to be viewable, as most bacteria are colourless and tend to blend into the background, it is therefore critical to provide a contrast between the microbes and their background.
Wiki User
∙ 11y agoWhen you look at bacteria through a microscope, it is hard to see much in the way of the different parts of them.
By adding a certain stain, like methylene blue, it makes the different parts of the bacteria show right up.
Wiki User
∙ 10y agoMany microorganisms are colorless and require staining in order to observe their morphology, arrangement, etc under microscopes. Without staining they are otherwise transluscent and are difficult to see without stain adding color to them.
Heat fixing (running the slide 3 times through the top of the Bunsen burner flame), initially kills the microorganism on the slide and helps adhere the microorganism to the slide. Without this method of "sticking", the microorganism may get washed off the slide during a staining procedure.
Wiki User
∙ 14y agoin order to see the cell clearly from the background when the microscope zooms in
Wiki User
∙ 13y agoYou stain cheek cells in order to see them.
Wiki User
∙ 12y agoTo identify unknown tissues and for health related issues
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Does the gram stain procedure work for all bacteria?
Yes. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive cells which do stain purple. In structural terms, the ability of a cell to become stained during the gram stain procedure is due to the chemical makeup of the cell wall.
Can you do a gram stain from 3 day old nutrient agar plate or should i do anohter t streak and let it grow for one day nd then do gram stain?
there should be no problem with doing a gram stain on a 3 days old bacteria strain unless your working with spore making bacteria, then you would need to do another streak, unless your familiar with spore stain methods but you should keep the bacteria cold at 4 degrees if you dont want them to die.
When treated with Gram stain Gram-positive bacteria appear?
Gram positive bacteria retain the violet stain, and appear purplish under a microscope.
Bacteria are divided into two groups based on how their cell walls absorb?
Stain.
What are the consequences of Ziehl-Neelsen staining?
The Ziehl-Neelsen stain is also known as the acid-fast stain. It contains sulfuric acid, and is used to identify acid-fast bacteria, or bacteria resistant to decolorization by acids from staining.
What is the gram stain of H5N1?
The Gram stain is used for bacteria and not for viruses.
What does gram negative on a serratia marcescens gram stain mean?
Bacteria are gram positive or gram negative. Serratia happens to be a gram negative bacteria. They appear pink on a gram stain. Gram positive bacteria stain to a purple color on a gram stain. We can classify and ID bacteria using their gram stain and shape. Some antibiotics only work on gram negative bacteria and some only work on gram positive bacteria. It helps a doctor know which antibiotic to use.
What is the different reaction of Gram-negative and Gram-positive bacteria to Gram's stain testing?
Gram-positive bacteria which take up the stain turn purple, while Gram-negative bacteria which do not take up the stain turn red.
Could a negatively charged dye stain a bacteria?
The effect on the bacteria depends if the stain is an acidic or basic stain. Most bacteria are stained when a basic stain permeates the cell wall and adheres by weak ionic bonds to the bacterial cell, which is slightly negatively charged.
What is the stain most useful in clinicians to decide for bacterial infection?
Methylene blue a basic stain is generally used to identify the external morphology of bacteria.The other stain which is used as differential stain and which can also differentiate the baceteia on the basis of their cell wall is gram stain i.e. Crystal voilet and is counter stained with Saffranine
Does the gram stain procedure work for all bacteria?
Yes. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive cells which do stain purple. In structural terms, the ability of a cell to become stained during the gram stain procedure is due to the chemical makeup of the cell wall.
Why does some bacteria stain purple and some red?
The purple stained bacteria are called gram positive bacteria and retain the stain after washing by having a thick cell wall one one layer of a heavily crossed linked material called peptidoglycan. ( Google that ) The red stained bacteria are gram negative because they posses two thinner layers of peptidogylcan cell wall that that do not hold the gram stain and it is mostly washed away in rinsing the bacterial samples.
Can you do a gram stain from 3 day old nutrient agar plate or should i do anohter t streak and let it grow for one day nd then do gram stain?
there should be no problem with doing a gram stain on a 3 days old bacteria strain unless your working with spore making bacteria, then you would need to do another streak, unless your familiar with spore stain methods but you should keep the bacteria cold at 4 degrees if you dont want them to die.
What color is Brilliant green K in Acid fast cell?
Acid-fast bacteria stain pink. Non acid-fast bacteria stain green.
When treated with Gram stain Gram-positive bacteria appear?
Gram positive bacteria retain the violet stain, and appear purplish under a microscope.
Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH affect the staining of bacteria?
# The pH will determine if the bacteria will have a particular charge. If the chromophore is a positive ion like the methylene blue in the equation shown in the reading, the stain is considered a basic stain; if it is a negative ion, it is an acidic stain. Most bacteria are stained when a basic stain permeates the cell wall and adheres by weak ionic bonds to the negative charges of the bacterial cell.
Which thing make gram positive bacteria to get stain?
the smooth part of the cell wall make it to get stain
