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Can you do a gram stain from 3 day old nutrient agar plate or should i do anohter t streak and let it grow for one day nd then do gram stain?
Wiki User
∙ 14y ago
there should be no problem with doing a gram stain on a 3 days old bacteria strain
unless your working with spore making bacteria, then you would need to do another streak, unless your familiar with spore stain methods
but you should keep the bacteria cold at 4 degrees if you dont want them to die.
Wiki User
∙ 14y ago
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What is a streak plate?
A streak plate is a surface of unglazed ceramic, used to find the true color of a mineral specimen by drawing the specimen across it. The color of the resultant powder is referred to as the streak or streak color of a mineral.
What will a streak plate with two species of bacteria look like?
A streak plate, with 2 species of bacteria, will show the bacteria in straight lines. Each species of bacteria will be separate from the other.
Why use a streak plate to grow a bacterium?
You do a streak plate in order to get isolated colonies. If you inoculate into a slant, you have less surface area to work and less chance of getting isolated colonies. In broth, you'll definitely get growth but you won't know WHAT is growing. You go back into each quadrant (a little) with your loop in order to "dilute" the bacteria and get colonies. Quadrant 1 is pretty think (like a smear on the plate) but by the time you get to Quadrants 3 and 4, you should see more defined colonies and not just a film of bacteria.
What is the differences between a streak plate technique and a serial dilution technique?
what is serial dilution and spread plate technique
What is the criteria for a good quality streak plate?
In scientific circles, the streak plate method is considered to be a rapid qualitative isolation method. To be effective, one must reduce the number of organisms in the inoculums by spreading a loop of culture over an agar plate. This ensures that individual cells are properly separated on the surface for the purpose of differentiating various species. The method is as follows: Using a sterile loop, microbes are initially transferred to the plate with one swipe. On the subsequent swipes, the loop is heated in the flame of a Bunsen burner to lessen the population of microbes being transmitted. Streak patterns are also done in via T-streak or by applying the loop to four quadrants of the plate.
When performing a streak plate in which direction should one rotate the nutrient plate?
anti clock wise
When performing a streak plate culture in what direction should one rotate the nutrient plate?
Counter-clockwise
What is the purpose of the plate?
A streak plate is used to determine the streak colour of a mineral. You take the mineral and scratch it against the streak plate, and it will create a coloured streak on the plate. The streak is not always the same colour as the mineral itself.
Will diamond leave a streak on a streak plate?
Diamond will not leave a streak on a porcelain streak plate because diamond is harder than the streak plate. It will leave a scratch on the streak plate for the same reason.
What happens if you rub the mineral across a streak plate but the mineral does not leave a streak?
The lack of a streak would indicate that the mineral is harder than the streak plate, or the color of the streak is the same as the color of the streak plate.
How do you test out a streak?
by strecting it on a streak plate
Can you show me a picture of a streak plate that has a streak from aquamarine on it?
I do not have a picture, but when you scratch aquamarine on a streak plate, the powder will be white colored.
Why do some very hard minerals leave no streak?
Some minerals are harder than a streak plate and will therefore leave no streak or the powder of the ceramic streak plate.
How do you determine the streak of a mineral whose Mohs scale is higher than the streak plate?
You can determine the streak of a mineral whose Mohs scale is higher than the streak plate by either filing or crushing with a hammer before rubbing the sample on a streak plate.
Why doesn't the mineral topaz leave a streak on a streak plate?
It leaves a scratch instead of a streak because Topaz has a higher number on the Moh's scale compared to the streak plate.
What is the name of the streak when you apply specimen to agar plate?
It is called a streak plate, inoculating the agar plate with a zig-zag motion.
When you rub a pumice rock on a streak plate what color is left on the streak plate?
The streak test is really only useful in mineral identification, not for combinations of them as with pumice.
