0
What is the name of the streak when you apply specimen to agar plate?
Wiki User
∙ 14y ago
It is called a streak plate, inoculating the agar plate with a zig-zag motion.
Wiki User
∙ 14y ago
Add your answer:
Earn +20 pts
What are the steps in properly streaking a plate?
When we have to isolate a specific microbial species from their mix culture or to grow any microbe on solid surface for their further studies then they can be grow on a culture medium containing a gel like substance known as agar which produce disticnt microbial colonies when inoculate in a petri dish containing the growth medium. The way by which the inoculation of microbial sample done is called a streak plate method in which the microbial sample is streak with the help of inoculating loop on the agar plate firmly, in the way so that the cell can be isolated. There are two more method to incoulate the microbial sample that are: pour plate and spread plate techniques.
Why is a TSA used for the streak plate?
Tryptic soya agar plates are used for streaking as it is a very general agar (ie not selective) and it promotes the growth of a broad spectrum of micoorganisms
Why do you invert a Agar plate when incubating?
1. What is the purpose of inverting inoculated plates during incubation?2. Where should colonies appear in the case of : a. Streak plate b. pour plates3. Indicate the temperature ranges for the following microbial categories.a. psychropiles b. mesophiles c. thermopiles4. What factors could account for an absence of growth on a pour plate?5. What factors could account for an absence of growth on a streak plate?6. What explanations could be given for the failure of obtaining isolated colonies on a streak plate?7. Gelatin and Agar comparison:a. Chemical composition b. temperature required for melting and solidifyc. Possibility of enzymatic attack by bacteria, Yes or No.
Why the inoculated agar medium plate must be inverted position in incubator?
Moisture in the air condenses on the lid of the plate and drops on top the agar if the plates are place right way up. The falling water droplets will spread the bacteria and especially ruin streak plates and spead plates where you need clear distict separate colonies.
What color is S marcescens in an agar slant tube?
Serratia marcescens makes a bright red streak on an agar slant
What is the significance of using a 'dry and hard' agar plate in streak plating?
Why is it impotant to use dry and hard agar for streaking
What are the steps in properly streaking a plate?
When we have to isolate a specific microbial species from their mix culture or to grow any microbe on solid surface for their further studies then they can be grow on a culture medium containing a gel like substance known as agar which produce disticnt microbial colonies when inoculate in a petri dish containing the growth medium. The way by which the inoculation of microbial sample done is called a streak plate method in which the microbial sample is streak with the help of inoculating loop on the agar plate firmly, in the way so that the cell can be isolated. There are two more method to incoulate the microbial sample that are: pour plate and spread plate techniques.
What happens when you prepare a smear from a agar plate or slant without first placing water on slide?
If you prepare a slide from an agar plate without placing water on the slide first the agar will be to lumpy on the slide. It will make to much of the specimen be in one spot.
Why is a TSA used for the streak plate?
Tryptic soya agar plates are used for streaking as it is a very general agar (ie not selective) and it promotes the growth of a broad spectrum of micoorganisms
Why are agar plates dried before being used for the preparation of dilution streak plates?
perhaps it is easier to streak that way, i mean when the agar is set and dry. .
Where should a label be written on an agar plate?
On the base of the agar plate.
Where should a label written on an agar plate?
On the base of the agar plate.
What does it mean to inoculate an agar plate?
to inoculate an agar plate you would place bacteria on the agar to grow
Why do you invert a Agar plate when incubating?
1. What is the purpose of inverting inoculated plates during incubation?2. Where should colonies appear in the case of : a. Streak plate b. pour plates3. Indicate the temperature ranges for the following microbial categories.a. psychropiles b. mesophiles c. thermopiles4. What factors could account for an absence of growth on a pour plate?5. What factors could account for an absence of growth on a streak plate?6. What explanations could be given for the failure of obtaining isolated colonies on a streak plate?7. Gelatin and Agar comparison:a. Chemical composition b. temperature required for melting and solidifyc. Possibility of enzymatic attack by bacteria, Yes or No.
What are some advantages of an agar plate over a slant tube?
The following are some advantages of an agar plate verses a slant tube: 1. Surface area- An agar plate has a much larger surface area: a. Easier to isolate individual colonies using the streak-plate method. i. Evaluate the colony shape, margin and elevation. b. Can grow a larger number of cells. 2. Growth- An agar plate allows you to quantify the number of colonies on an agar plate, provided it is within the 30-300 range. Whereas the slant tube cannot quantify growth but only describes growth as none, slight, moderate, or large.
How do the results of pour plate method compare with those obtained using the streak plate and spread plate method?
In the pour plate, the microorganisms will grow within the gel that has been set, and in the spread-plate technique, growth will be on top of the agar gel where it has been spread.
What is isolation media in microbiology?
The growth media such as nutrient agar or Mckonkey agar is used to isolate microbial cells from the mix culture when inoculate on them. Some time speical media is used which selectivly grow some time of bacteria or a differential media which give different morphology of different bacterial species e.g. blood agar and Mannitol salt agar. It can be done by three ways: the spread plate method, the streak plate method, and the pour plate method.
