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higly unretainable and has high absorption at 260 nm
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Which nitrogen base is not used during replication?
Uracil. Uracil is not present in DNA, but it is present in RNA. DNA's "equivalent" base is thymine, meaning when DNA is transcribed into RNA, the places where thymine would go instead has uracil.
What is the base of RNA in place of thymine which bonds with adenine?
The nitrogen base uracil takes the place of thymine in RNA. So in RNA, uracil pairs with adenine.
Is uracil an enzyme?
No. Uracil is a Pyrimidine.
3 DNA does NOT contain the nitrogen base?
DNA does not contain uracil. RNA does!! DNA contains guanine binds with Thymine in DNA RNA contains guanine that binds with uracil DNA does not contain uracil. RNA does!! DNA contains guanine binds with Thymine in DNA RNA contains guanine that binds with uracil
What nitrogen base replaces thymine (T) in RNA?
RNA and DNA both share the nitrogen bases adenine (A), cytosine (C), and guanine (G).
Why uracil used in hplc calibration?
higly unretainable and has high absorption at 260 nm
Why is Erbium perchlorate is used in calibration of HPLC?
Erbium has a strong absorption in uv and visible range, It is used in HPlc calibration for the wavelength accuracy verification of the PDA detector.
Why propyl paraben used in HPLC calibration?
propyl paraben being a stable compound with reproducible chromatographic parameters used as a internal standard for the calibration of the HPLC systems
What can one read in the HPLC Column?
In an HPLC column one can see very small molecules such as ATP, histidine, glucose, uracil, and pyridine. It is a form high quality of liquid Chromatography.
What are the HPLC Calibration parameters and elaborate it?
1. Flow rate 2. Temp. of column 3. Detector function 4. Resolution
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
Why internal standard you used in hplc method?
Internal standard can be used for calibration by plotting the ratio of the analyte signal to the internal standard signal as a function of the analyte concentration of the standards. This is done to correct for the loss of analyte during sample preparation or sample inlet.
Definition of standard in HPLC?
Answer: HPLC standards are an indispensible tool for analytical HPLC applications. They are used to monitor column performance & calibrate detector response.
When calibration required?
Calibration is required during installation of new instrument to check whether the instrument works as per specifications or not. Calibration is required when instrument is shifted from one location to another. Calibration of instrument is done when instrument is used again after maintenance. Some instruments such as weighing balance are calibrated daily while some instruments or equipments such as HPLC, GC, etc., are calibrated or validated at specified interval as per standard operating procedure (SOP).
What is difference bet GLC and HPLC?
GLC has a stationary liquid phase and gas moving phase HPLC had a stationary solid phase and liquid moving phase HPLC is done under high pressure. HPLC can be used for thermally unstable compounds as opposed to GLC HPLC can be used for polar or low volatile compounds as opposed to GLC
How does one apply the HPLC princple?
The HPLC principle is used to separate liquid mixtures into individual parts. It's frequently used in several field such as medicine and law. HPLC is specifically used in sports to test for performance enhancing drugs in urine.
When are calibration standards used?
Calibration standards are used to record the accuracy of certain calibrations that are performed. The accuracy allows the user to find out how good the calibration was.
