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1. Flow rate
2. Temp. of column
3. Detector function
4. Resolution
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What is rs-hplc?
"RS-HPLC method" means "Related Substance HPLC Method".
What is RRT and RRF in hplc?
In HPLC RRT means Relative Retention Time and RRF is Relative Response Factor
What is an HPLC column?
HPLC Column is one type of tube containing a stationary phase react with mobile phase to detect peak
What is the definition of method development by hplc?
Method development is a process amenable to continuous improvement
What are process parameters?
A process parameter refers to the current status of a procedure under control. It is also known as a process variable or process value.
Why propyl paraben used in HPLC calibration?
propyl paraben being a stable compound with reproducible chromatographic parameters used as a internal standard for the calibration of the HPLC systems
Why is Erbium perchlorate is used in calibration of HPLC?
Erbium has a strong absorption in uv and visible range, It is used in HPlc calibration for the wavelength accuracy verification of the PDA detector.
Why uracil used in hplc calibration?
higly unretainable and has high absorption at 260 nm
Why uracil is used in hplc calibration?
higly unretainable and has high absorption at 260 nm
How do you calibrate for HPLC?
standards are run with samples i.e. several solutions of chemical you are trying to analyse for, of known composition and strengths are run to set up a calibration curve which should be a straight line - absorbance (or signal strength) vs. conc. You then test the unknown sample and can extraploate the concentration of the sample based on your calibration curve. HPLC columns come with a standard chromatogram when purchased so a run with same conditions and sample should give similar retention times.
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
Which one is advanced gc or hplc?
HPLC is advanced
When calibration required?
Calibration is required during installation of new instrument to check whether the instrument works as per specifications or not. Calibration is required when instrument is shifted from one location to another. Calibration of instrument is done when instrument is used again after maintenance. Some instruments such as weighing balance are calibrated daily while some instruments or equipments such as HPLC, GC, etc., are calibrated or validated at specified interval as per standard operating procedure (SOP).
Retention time calculation for hplc?
why RT was shifting & how to RT calculation in HPLC
What is C8 and C18?
HPLC columns. (HPLC - High Performance Liquid Chromatography.)
Definition of standard in HPLC?
Answer: HPLC standards are an indispensible tool for analytical HPLC applications. They are used to monitor column performance & calibrate detector response.
Why internal standard you used in hplc method?
Internal standard can be used for calibration by plotting the ratio of the analyte signal to the internal standard signal as a function of the analyte concentration of the standards. This is done to correct for the loss of analyte during sample preparation or sample inlet.
