increasing the agarose concentration will enable the separation of smaller fragments of DNA. the structure of the gel (agarose) consists of crosslinks, therefore the higher the concentration of agarose the more crosslinks there will be and smaller size "holes" for the DNA to travel through (also the other way around, with less concentrated agarose)
The concentration of agarose will affect the density of the gel. A higher density will provide a greater number of obstacles, and therefore resistance, to the flow of DNA fragments.
This is used to adjust the 'transit time'. Smaller DNA fragments in a high concentration agarose gel will travel the same speed as larger fragments in a lower concentration gel.
No, the agarose gel is just a polysaccharide.
to vizualise DNA after Agarose gel electrophoresis
Agarose gel electrophoresis is the easiest and most common method used in biochemistry and molecular biology in separating DNA or RNA molecules according to their size.
The process is referred to as gel electrophoresis. This is an analytical process where DNA fragments can be separated based on size within a gel under the influence of an electric field
Agarose gel electrophoresis is for determining the size of a piece of RNA or DNA. It can be used to determine the culprit of a crime, relatives, even the cause of some diseases, like Mad Cow.
Agarose gel electrophoresis is suitable for ALL DNA.
Check the answer for How do you make an electrophoresis gel?
For larger molecules like proteins we use polyacrylamide gel electrophoresis (PAGE). For smaller pieces like DNA we use agarose gel electrophoresis
agarose helps in the separation of DNA bands by controlling the pore size of agarose gel
No, the agarose gel is just a polysaccharide.
eating
A. J. Houtsmuller has written: 'Agarose-gel-electrophoresis of lipoproteins' -- subject(s): Blood protein electrophoresis, Electrophoresis, Gel electrophoresis, Lipoproteins
it is a technique that separated dna according to its size.
to vizualise DNA after Agarose gel electrophoresis
Agarose is used in gel electrophoresis to separate nucleic acids (like DNA) by size, charge an other physical properties. Gel electrophoresis uses an electrical current to make particles move. For example, DNA is negative, so it'll travel towards to positive electrode of the gel box. Agarose has small pores through which a DNA can travel. Bigger fragments of DNA travel shorter distances, because it takes longer for them to navigate through the pores of the agarose gel. Identically sized pieces of DNA will travel the same distance, which is why you get bands (DNA with loading dye) after you run a a gel.
Agarose gel electrophoresis is the easiest and most common method used in biochemistry and molecular biology in separating DNA or RNA molecules according to their size.
The larger fragements will not be very accurate because they cannot resolve in high consentrations of the agarose in the gel. The percent of agarose in the gel affects the ability to resolve larger fragements of DNA