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Primer extension method

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Q: A nick in DNA can be detected by which method?
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A nick in a DNA molecule can be detected by which of the following methods?

primer extension technique


What parts of DNA molocules are the basis detected by DNA fingerprinting?

Links between strands


What is meant by hot and cold radio-isotope labeling with reference to DNA sequencing?

What they mean by "hot" is when the substance is "radioactive" and as such it could be detected by an X-ray film. I am not to sure with the "cold" method.


How is Fragile X syndrome detected?

By a DNA blood sample


When new DNA molecules are formed almost errors are detected and fixed by?

Errors are fixed by DNA polymerase...


What is difference between Qualitative PCR and Quatitative PCR?

In qualitative PCR specific DNA fragment is detected while in quantitative PCR our target DNA sequence not only is detected but its amount is determined (after reaction we can calculate the amount of DNA we had in our sample)


How is DNA tested and why?

by DNA fingerprinting method , DNA-DNA hybirdization or DNA sequencing. to know the sequence of DNA


What is a method of making any copies of DNA?

Polymerase chain reaction (PCR) is the method of making copies of DNA.


Where do you get the DNA fragments for Gel electrophoresis?

The DNA fragments comes from the method of DNA isolation.


NOT a likely method of DNA replication as determined by the structure of DNA?

despesive


What is the diffecence between sensitivity and detection limit?

Sensitivity is the smallest change in concentration of the analyze that can be detected by using that method. This is the slope of the calibration curve. Detection limit is the lowest concentration that can be detected by the particular method.


What is maxam Gilbert method of DNA packaging?

Used in DNA sequencing; four samples of end-labeled DNA restriction fragments are chemically cleaved at different specific nucleotides. The resulting subfragments are separated by gel electrophoresis, and the labeled fragments are detected by autoradiography. The sequence of the original end-labeled restriction fragment can be determined directly from parallel electropherograms of the four samples