Primer extension method
Links between strands
What they mean by "hot" is when the substance is "radioactive" and as such it could be detected by an X-ray film. I am not to sure with the "cold" method.
Errors are fixed by DNA polymerase...
The DNA fragments comes from the method of DNA isolation.
despesive
primer extension technique
Links between strands
What they mean by "hot" is when the substance is "radioactive" and as such it could be detected by an X-ray film. I am not to sure with the "cold" method.
By a DNA blood sample
Errors are fixed by DNA polymerase...
In qualitative PCR specific DNA fragment is detected while in quantitative PCR our target DNA sequence not only is detected but its amount is determined (after reaction we can calculate the amount of DNA we had in our sample)
by DNA fingerprinting method , DNA-DNA hybirdization or DNA sequencing. to know the sequence of DNA
Polymerase chain reaction (PCR) is the method of making copies of DNA.
The DNA fragments comes from the method of DNA isolation.
despesive
Sensitivity is the smallest change in concentration of the analyze that can be detected by using that method. This is the slope of the calibration curve. Detection limit is the lowest concentration that can be detected by the particular method.
Used in DNA sequencing; four samples of end-labeled DNA restriction fragments are chemically cleaved at different specific nucleotides. The resulting subfragments are separated by gel electrophoresis, and the labeled fragments are detected by autoradiography. The sequence of the original end-labeled restriction fragment can be determined directly from parallel electropherograms of the four samples