Gram positive: dark purple
Gram negative: light pink
You may need to do your gram stain over again. It should be either dark purple or light pink.
Crystal violet and safranin are functionally analogous pair of stains in the Gram staining technique. Crystal violet stains gram-positive bacteria purple/blue, while safranin counterstains gram-negative bacteria pink/red.
RED GRAM means KANDI PAPPU
Neither - the influenza VIRUS is not typed by gram stains - only bacteria are.
Escherichia coli (E. coli) cells typically appear as Gram-negative rods under a Gram stain. This means that they will appear pink or red after staining due to the thin layer of peptidoglycan in their cell walls that does not retain the crystal violet stain.
E. coli is a gram negative bacteria, meaning that it has a cytoplasmic lipid membrane, a peptidoglycan layer, and a (LPS) lipopolysaccharide layer. As a result, e. coli stains a pink colour on a gram stain from the counterstain saffranin. Gram positives stain purple retain the crystal violet dye even after washed with a decolouring solution.
If you are talking about a Gram Stain, then red. E. coli is Gram negative which means that Safranin will stain it red during a gram stain.
A
safranin
Gram-negative bacteria Gram-negative bacteria have an outer membrane. However, they have a thinner peptidoglycan cell wall. This means they do not hold the blue dye used in Gram testing and do not appear blue. Instead, they appear red or pink in color.
Its the primary stain of the procedure. IT stains the Gram positive organisms
During the procedures of a gram stain, decolorization is necessary to remove any stain or color from the gram negative cells. When a dye is used to stain gram positive cells, both gram positive and gram negative cells retain color. Mordant is used to bind the original stain to gram positive cells so when decolorizer is used they retain color. After the mordant has been used a decolorizer is used to wash away colo in gram negative cells. Counterstains are used to stain gram negative cells to better visualize contrasting cells. An example of a decolorizer that works well is ethanol.
S. mitis stains purple and therefore is a gram positive bacteria