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This depends on the strength of the filtration system on the tank. On a 'normal' five gallon tank (filter turnover about 5x/hour) three or four would be a good number. On a 'five gallon tank on steroids' (I have one of these - filter turnover 12x/hour) I kept seven with no problems. If you're going to keep more than three or four, regardless of your filtration, you need to be religious about tank maintenance - 50% water change, every week. Keeping more than 6 or 7 in the tank is probably not going to work.

(For filter turnover, 5x/hour means the filter pumps 5 times the tank's volume each hour, so that would be a 25gph filter in a 5 gal tank.)

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Q: About how many guppys can live a 5 gallon fish tank?
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