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You can use Isoamyl alcohol instead of octanol. both of them are anti-foaming agents and facilitate separation of phases after centifugation.
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Different between CTAB method and Dellaporta method?
In CTAB method,SEVAG is used to breakdown the tissues in the extracted leaves.While in dellaporta method,the SDS and POTASSIUM ACETATE are used.In CTAB method BLUECAP/TEST TUBES are used,while in dellaporta method the EMPENDORFS are mostly used.ICE COLD ETHANOL is used mostly in the CTAB method for resuspension,while in dellaporta method ISOPROPANOL is used.
What is the importance of ctab buffer?
importance of ctab buffer
Why use CTAB in DNA isolation?
The CTAB extraction procedure is from Rogers and Bendich (1986). The magic bullet is supposed to be the separation of polysaccharides from nucleic acids by the use of CTAB. The technique capitalizes on the previous observations that nucleic acids can be selectively precipitated with CTAB. RNA and DNA are soluble in CTAB and 0.7 M NaCl but precipitate when the salt is reduced below 0.4 M. However, many polysaccharides are insoluble over this salt range and are thus not solubilized. CTAB is NOT used to lyse membranes in this procedure.
Role of ctab in isolating DNA from soil sample?
CTAB is a surfactant used in the isolation of DNA from tissues containing high amounts of polysaccharides. Under the high-salt conditions of this protocol, CTAB binds the polysaccharides removing them from the solution. When combined with Arabidopsis, this procedure yields pure DNA.
Structure provides one alternative path of execution?
A sequence structure
Different between CTAB method and Dellaporta method?
In CTAB method,SEVAG is used to breakdown the tissues in the extracted leaves.While in dellaporta method,the SDS and POTASSIUM ACETATE are used.In CTAB method BLUECAP/TEST TUBES are used,while in dellaporta method the EMPENDORFS are mostly used.ICE COLD ETHANOL is used mostly in the CTAB method for resuspension,while in dellaporta method ISOPROPANOL is used.
What is the difference between CTAB and SDS method?
This refers to the type of detergent used to lyse cell membranes when extracting DNA from cells. SDS=Sodium dodecyl sulfate, CTAB=Cetyl trimethylammonium bromide
What is the importance of ctab buffer?
importance of ctab buffer
Isolation of genomic DNA from groundnut leaves?
To isolate genomic DNA from groundnut leaves, you can use a commercial DNA extraction kit designed for plant tissue. Follow the kit's protocol, which usually involves grinding the leaves in a lysis buffer, isolating the DNA through purification steps, and then eluting the DNA in a suitable buffer. Ensure that you properly store the extracted DNA at -20°C to -80°C for later use.
Why use CTAB in DNA isolation?
The CTAB extraction procedure is from Rogers and Bendich (1986). The magic bullet is supposed to be the separation of polysaccharides from nucleic acids by the use of CTAB. The technique capitalizes on the previous observations that nucleic acids can be selectively precipitated with CTAB. RNA and DNA are soluble in CTAB and 0.7 M NaCl but precipitate when the salt is reduced below 0.4 M. However, many polysaccharides are insoluble over this salt range and are thus not solubilized. CTAB is NOT used to lyse membranes in this procedure.
What is the molar heat of combustion of 1-octanol?
The molar heat of combustion of 1-octanol is approximately -6,268 kJ/mol. This value represents the amount of heat released when one mole of 1-octanol is completely burned in excess oxygen to form carbon dioxide and water.
Why is Octanol an energy efficient fuel?
Octanol is considered an energy efficient fuel due to its high energy density, which is approximately 30% higher than gasoline. This means that Octanol can provide more energy per unit volume, allowing it to generate more power and efficiency when used as a fuel for engines or vehicles. Additionally, Octanol has a lower risk of evaporation compared to other biofuels, reducing energy losses during storage and transportation.
What is the smell of octanol?
Octanol does not have a specific smell as it is a chemical compound. However, some people may describe the smell of octanol as slightly floral, fruity, or waxy. It is important to note that individual perceptions of smell can vary.
Role of ctab in the lysis of cell membrane?
none
Is n-octane polar?
water is polar and immiscible with the non-polar octanol.
Is octanol soluble in water?
Octanol is sparingly soluble in water because of its hydrophobic nature. The long hydrophobic carbon chain in octanol is not favorable for interaction with water molecules, preventing it from dissolving easily in water.
Role of CTAB in DNA isolation?
It sequester carbohydrates in the solution
