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You can use Isoamyl alcohol instead of octanol. both of them are anti-foaming agents and facilitate separation of phases after centifugation.
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Different between CTAB method and Dellaporta method?
In CTAB method,SEVAG is used to breakdown the tissues in the extracted leaves.While in dellaporta method,the SDS and POTASSIUM ACETATE are used.In CTAB method BLUECAP/TEST TUBES are used,while in dellaporta method the EMPENDORFS are mostly used.ICE COLD ETHANOL is used mostly in the CTAB method for resuspension,while in dellaporta method ISOPROPANOL is used.
What is the importance of ctab buffer?
importance of ctab buffer
Why use CTAB in DNA isolation?
The CTAB extraction procedure is from Rogers and Bendich (1986). The magic bullet is supposed to be the separation of polysaccharides from nucleic acids by the use of CTAB. The technique capitalizes on the previous observations that nucleic acids can be selectively precipitated with CTAB. RNA and DNA are soluble in CTAB and 0.7 M NaCl but precipitate when the salt is reduced below 0.4 M. However, many polysaccharides are insoluble over this salt range and are thus not solubilized. CTAB is NOT used to lyse membranes in this procedure.
Role of ctab in isolating DNA from soil sample?
CTAB is a surfactant used in the isolation of DNA from tissues containing high amounts of polysaccharides. Under the high-salt conditions of this protocol, CTAB binds the polysaccharides removing them from the solution. When combined with Arabidopsis, this procedure yields pure DNA.
What are the method of controlling panthogen?
what are the method of controlling panthogens what are the method of controlling panthogens what are the method of controlling panthogens
Different between CTAB method and Dellaporta method?
In CTAB method,SEVAG is used to breakdown the tissues in the extracted leaves.While in dellaporta method,the SDS and POTASSIUM ACETATE are used.In CTAB method BLUECAP/TEST TUBES are used,while in dellaporta method the EMPENDORFS are mostly used.ICE COLD ETHANOL is used mostly in the CTAB method for resuspension,while in dellaporta method ISOPROPANOL is used.
What is the difference between CTAB and SDS method?
This refers to the type of detergent used to lyse cell membranes when extracting DNA from cells. SDS=Sodium dodecyl sulfate, CTAB=Cetyl trimethylammonium bromide
What is the importance of ctab buffer?
importance of ctab buffer
Isolation of genomic DNA from groundnut leaves?
Hello, Its very simple, u can go for the CTAB method , with bit modification, 1) Ensure the youngest possible leaves, very tender enough to crush. 2) Crush the leaf sample in liquid nitrogen. 3) add quickly CTAB buffer to the crushed isolate immediately. 4) the follow Rapid method of isolation.
Why use CTAB in DNA isolation?
The CTAB extraction procedure is from Rogers and Bendich (1986). The magic bullet is supposed to be the separation of polysaccharides from nucleic acids by the use of CTAB. The technique capitalizes on the previous observations that nucleic acids can be selectively precipitated with CTAB. RNA and DNA are soluble in CTAB and 0.7 M NaCl but precipitate when the salt is reduced below 0.4 M. However, many polysaccharides are insoluble over this salt range and are thus not solubilized. CTAB is NOT used to lyse membranes in this procedure.
What is the molar heat of combustion of 1-octanol?
The molar heat of combustion of octanol is 5294
Why is Octanol an energy efficient fuel?
"Octanol is not the most energy efficient fuel. Butanol is. Octanol gives out 8691.34J of energy per gram. Butanol gives out 15521.21J of energy per gram" Please state your source.
What is the smell of octanol?
you tell me
Role of ctab in the lysis of cell membrane?
none
Is n-octane polar?
water is polar and immiscible with the non-polar octanol.
Is octanol soluble in water?
no it isn't. Since the molecule has over 7 carbon atoms it will not dissolve. Also, the Solubility of octanol is really small.
Role of CTAB in DNA isolation?
It sequester carbohydrates in the solution
