In 1970s, due to clear felling of forests in Brazil and Central America, there was a shortage of logwood and therefore of haematoxylin. Its price went to record heights, which affected the cost of diagnostic histopathology, and prompted a search for alternative nuclear stains. Before the use of any alternatives became firmly established, haematoxylin returned to the market, though at a higher price, and resumed its place in histopathology. There were several dyes recommended as replacements: Celestine blue B (CI 51050), Gallocyanin (CI 51030), Gallein (CI 45445) and Solochrome cyanin (CI 43820). All four used Fe(III) as the mordant. Another alternative is the red dye brazilin, which differs from haematoxylin by only one hydroxyl group for eosine alternate you can ues other compound,There are actually two very closely related compounds commonly referred to as eosin. Most often used is eosin Y (also known as eosin Y ws, eosin yellowish, Acid Red 87, C.I. 45380, bromoeosine, bromofluoresceic acid, D&C Red No. 22); it has a very slightly yellowish cast. The other eosin compound is eosin B (eosin bluish, Acid Red 91, C.I. 45400, Saffrosine, Eosin Scarlet, or imperial red); it has a very faint bluish cast. The two dyes are interchangeable, and the use of one or the other is a matter of preference and tradition
Eosin stains the cytoplasm of the cell pink-orange.
Eosin and methylene blue are used for staining biolgical tissues.
blue staining of connective tissues when haematoxylin-eosin stain is used; found in such conditions assolar elastosis.
To make the Eosin Methylene BLue Agar look cool and hip.
function of eosin methylene blue agar (EMB)
Hematoxylin is only the drastic substance that these solutions contain. For histology, the two most commonly used are Mayer's Hematoxylin and Harris' Hematoxylin. They both contain water, hematoxylin and various salts.
HAEMATOXylin colors nuclei blue while EOSIN colors tissues, cells, or organelles pink and it also binds to proteins which makes H & E stain combination very successful in identifying structures of tissues, nuclei, cells, etc
Eosin stains the cytoplasm of the cell pink-orange.
To stain or dye tissues (animals or plants).
what are the types of haematoxylin stains and its applications
Eosin is a red stand and methylene blue is blue. The result of staining a bacterial smear with a mixture of eosin and methylene blue is that eosin is acidic and acts as a negative stain. Methylene blue is basic the smear background would turn out red while the cells would turn out blue.
A general term for a chemical that makes a specimen visible is a stain. There are many types of stains available, depending upon the structure you want to visualize and the type of microscope you want to use, e.g. fluorescent stains like DAPI for fluorescence microscopy, or hematoxylin and eosin (H and E) staining for brightfield microscopy.Immersion oil
Polychromed methylene blue Eosin Azure Leishman powder Alcohol free absolute alcohol
Eosin and methylene blue are used for staining biolgical tissues.
It depends on what tissue you're looking at, what you want to stain, how the tissue has been stored... Besides very specific staining, there are different types of staining. For example, immunohistochemistry, which uses antibodies to stick coloured stains to cell surface receptors. Or, chemical staining - the most common is H&E staining (haemotoxylin & eosin), so if you're just having fun in a lab and want to see general structures of cells, use this one.
A stain which isappliedto the dead cells are known as non vital stain for example eosin. aniline blue, orange G etc.
a stain employed to detect amyloid, which induces specific yellow fluorescence; tissue sections are first put in alum-hematoxylin to quench nuclear fluorescence and then stained in thioflavine T.