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Is mycobacterium phlei gram positive or negative?
Although mycoplasma lack a cell wall and therefore test gram negative, they are considered to be descendents of 'nonsporulating and endospore forming gram-positive bacteria' (Madigan et al., 2009), such as Lactobacilli, Bacilli and Streptococci, (Dandekar et al., 2002), which have lost their cell wall. Mycoplasmas are therfore classed as gram- positive bacteria. This is supported by ribosomal RNA and DNA analysis.
Difference between the gram stain ans acid fast stain reactions of E. coli?
Gram staining is useful in separating bacteria into two groups: Gram positive or Gram negative. They are separated into these groups based on their cell wall structure. Gram positive bacteria contain a thick layer of peptidoglycan in their cell walls, while Gram negative bacteria contain a very small layer of peptidoglycan (15% or less of what Gram positive cell walls contain). A primary stain is added, such as Crystal Violet, that will stain all of the bacteria. Then, a mordant (such as iodine and potassium iodide) is added to form a complex between the peptidoglycan and the stain, which will make the cell more resistant to decolorization. Then, a decolorizing agent is added, which will remove the primary stain from Gram negative bacteria, but will cause the cell walls in Gram positive bacteria to dehydrate, and therefore, they will retain the primary stain. Finally, a counterstain (typically safranin) is added to distinguish Gram positive from Gram negative. Gram positive cells will be purple, and Gram negative cells will be red if Crystal Violet and Safranin are used.Acid-fast staining is entirely different. Is is used to detect species of bacteria in the genera Mycobacteria and Nocardia. These bacteria are resistant to typical staining procedures, such as Gram staining, due to a thick, waxy lipid layer in the cell wall composed of mycolic acid. Heating of the bacteria with a very strong stain such as carbol-fuchsin is necessary to "melt" this lipid layer, and force the stain through the cell wall. Once the bacteria has cooled, they will be incredibly resistant to decolorization. Non-acid fast bacteria do not contain this mycolic acid layer, and therefore, they will decolorize much easier, and are then stained with a counterstain to distinguish Acid-Fast bacteria from Non-Acid-Fast bacteria.
Would an acid fast organism be gram positive or gram negative?
Acid fast organism would be gram positive.----------------------------------------------------------------------------------------------------The above answer is incorrect. Acid-fast bacteria would be "gram negative."Bacteria such as these have very different cell walls which contain a great deal of waxy material, although they still contain peptidoglycan. This waxy material prevents stain penetration, rendering the cell invisible.Since these acid-fast bacteria are not stained by Gram's Stain Method they have been characterized as "gram negative."----------------------------------------------------------------------------------------------------Heres what the text book says. This is a tricky question based on opinion."Their cell walls are unique among the gram-positive bacteria because they consist of other layers in addition to peptidoglycan. An important component of their walls is a group of fatty acids called mycolic acids. Some have proposed that the mycolic acids form a bilayer structure that is analogous to the outer membrane of gram-negative bacteria."So answer one is technically correct but they characteristics as those of gram negative cells (answer 2).Page 58Prescott's MicrobiologyEdition: 8thAuthor(s):Willey; Sherwood; WoolvertonISBN13:9780073375267ISBN10:0073375268Format:HardcoverPub. Date:1/1/2010Publisher(s):MCGRAW HILL
What are results for a acid-fast test for enterobacter aerogenes?
Enterobacter aerogenes is a non-acid fast bacteria. Bacteria only in the Mycobacteria and Nocardia genus are acid fast.
Are endospores acid fast?
No, endospores are not acid fast because they are not cells. Endospores will stain with the acid fast stain and look fuchsia but they are not positive for acid fast.
What kind of bacteria can harm animals?
Just about any - gram negative, gram positive and acid-fast are all known to have pathogenic bacteria within them.
Cite the purpose of following reagents in a differential staining procedure?
To distinguish between acid fast positive and acid fast negative bacteria. Acid fast positive bacteria will stain red to pink color and acid fast negative bacteria will stain blue. Acid fast positive bacteria have mycolic acid in their cell wall, which will stain with carbol fuchsin and not decolorize with acid alcohol. Acid fast negative bacteria do not have mycolic acid in their cell wall, and become decolorize with the acid alcohol. Counterstain of methylene blue needs to be done in order to see the acid fast negative.
What gram reaction do you expect from acid-fast bacteria?
Gram positive
Is mycobacterium phlei gram positive or negative?
Although mycoplasma lack a cell wall and therefore test gram negative, they are considered to be descendents of 'nonsporulating and endospore forming gram-positive bacteria' (Madigan et al., 2009), such as Lactobacilli, Bacilli and Streptococci, (Dandekar et al., 2002), which have lost their cell wall. Mycoplasmas are therfore classed as gram- positive bacteria. This is supported by ribosomal RNA and DNA analysis.
How does acid-fast bacteria differ from gram bacteria?
Acid-fast and Gram stain are two different types of staining technique. Bacteria that stain with acid-fast techniques are called "acid fast bacteria." Bacteria that don't stain with acid-fast techniques are called "not acid fast." At the same time, bacteria can be "Gram-positive" or "Gram-negative." For example, Nocardia is a bacterium that is acid-fast and Gram-positive. Usually the reason people care about these designations is in order to figure out what species a bacteria is. The issue usually arises in the context of a patient's sample that has grown bacteria. At first the doctors won't be sure which bacteria it is. They'll have guesses based on how the patient presented, but they won't know, and they want to know because that will help them pick the best treatment. A first step toward "speciating" the bacteria is to do a Gram stain on it. This is because for historical reasons determining whether a bacteria is Gram-positive or Gram-negative goes a long way toward speciating it. Acid-fast staining is less common, and is used mostly for diagnosing tuberculosis (caused by Mycobacteria, which are acid-fast bacteria). There are details about the cell walls that determine whether an organism will stain with Gram stain (thick walls without mycolic acids) or acid-fast, but hopefully this answers your question.
Is Micrococcus luteus acid fast negative?
Micrococcus leteus is negative to VP test.
Is the mycobacterium smegmatis positive to acid-fast?
NO, s. epidermidis does not show up positive in an acid fast stain. The acid fast stain is a differential stain that differentiates cells with mycolic acids in their cells walls with those who do not. cells with mycolic acid are therefore acid fast. s epidermidis does not contain this waxy substance in their cell wall so it doesnt fall into this category.
Difference between the gram stain ans acid fast stain reactions of E. coli?
Gram staining is useful in separating bacteria into two groups: Gram positive or Gram negative. They are separated into these groups based on their cell wall structure. Gram positive bacteria contain a thick layer of peptidoglycan in their cell walls, while Gram negative bacteria contain a very small layer of peptidoglycan (15% or less of what Gram positive cell walls contain). A primary stain is added, such as Crystal Violet, that will stain all of the bacteria. Then, a mordant (such as iodine and potassium iodide) is added to form a complex between the peptidoglycan and the stain, which will make the cell more resistant to decolorization. Then, a decolorizing agent is added, which will remove the primary stain from Gram negative bacteria, but will cause the cell walls in Gram positive bacteria to dehydrate, and therefore, they will retain the primary stain. Finally, a counterstain (typically safranin) is added to distinguish Gram positive from Gram negative. Gram positive cells will be purple, and Gram negative cells will be red if Crystal Violet and Safranin are used.Acid-fast staining is entirely different. Is is used to detect species of bacteria in the genera Mycobacteria and Nocardia. These bacteria are resistant to typical staining procedures, such as Gram staining, due to a thick, waxy lipid layer in the cell wall composed of mycolic acid. Heating of the bacteria with a very strong stain such as carbol-fuchsin is necessary to "melt" this lipid layer, and force the stain through the cell wall. Once the bacteria has cooled, they will be incredibly resistant to decolorization. Non-acid fast bacteria do not contain this mycolic acid layer, and therefore, they will decolorize much easier, and are then stained with a counterstain to distinguish Acid-Fast bacteria from Non-Acid-Fast bacteria.
What color is Brilliant green K in Acid fast cell?
Acid-fast bacteria stain pink. Non acid-fast bacteria stain green.
Would an acid fast organism be gram positive or gram negative?
Acid fast organism would be gram positive.----------------------------------------------------------------------------------------------------The above answer is incorrect. Acid-fast bacteria would be "gram negative."Bacteria such as these have very different cell walls which contain a great deal of waxy material, although they still contain peptidoglycan. This waxy material prevents stain penetration, rendering the cell invisible.Since these acid-fast bacteria are not stained by Gram's Stain Method they have been characterized as "gram negative."----------------------------------------------------------------------------------------------------Heres what the text book says. This is a tricky question based on opinion."Their cell walls are unique among the gram-positive bacteria because they consist of other layers in addition to peptidoglycan. An important component of their walls is a group of fatty acids called mycolic acids. Some have proposed that the mycolic acids form a bilayer structure that is analogous to the outer membrane of gram-negative bacteria."So answer one is technically correct but they characteristics as those of gram negative cells (answer 2).Page 58Prescott's MicrobiologyEdition: 8thAuthor(s):Willey; Sherwood; WoolvertonISBN13:9780073375267ISBN10:0073375268Format:HardcoverPub. Date:1/1/2010Publisher(s):MCGRAW HILL
What are results for a acid-fast test for enterobacter aerogenes?
Enterobacter aerogenes is a non-acid fast bacteria. Bacteria only in the Mycobacteria and Nocardia genus are acid fast.
Two diseases caused by acid fast bacteria?
Tuberculosis and leprosy are two diseases caused by acid fast bacteria.
