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The bacteria present in curd are primarily Gram-positive bacteria. These include species like Lactobacillus acidophilus and Streptococcus thermophilus. Gram-negative bacteria are not typically found in curd as the fermentation process favors the growth of Gram-positive bacteria.
AnswerYou can use a "selective" medium that will inhibit the growth of Gram positive bacteria and only allow Gram negative bacteria to multiply. A medium which is commonly used for this is the McConkey agar which contains a crystal violet strain and allows only Gram negative cultures to grow.You can also eliminate Gram positive bacteria with antibiotics (e.g. ampicillin) provided that they are sensitive and not resistant.
The gram positive cell wall contains a thicker layer of peptydoglacans than that of gram negative. the gram negative bacteria doesn't have a LPS(Lipopolysaccharides) covering outside the cell wall like the gram negatives.their is a periplasmal space between peptidoglycan cell wall and the cell membrane. During Gram's staining the CVI complex doesnt leave the gram positive bacteria because of the thick layer of peptydoglycanes.
Bacteria are classified in many ways. Most bacteria are classified by their shape, cell wall, movement, eating, and breathing. The most useful technique of classification would be the Gram stain. The Gram stain distinguishes between differences in the cell wall composition of bacteria (Gram positive or Gram negative). The shape of the bacteria could be spherical (coccus), rod (bacillus), spirochete, or vibrio (curved rod). The texture could be wet/moist, dry, or mucoid. Bacteria come in a wide variety of colors.
To determine which pathogen you are dealing with. Bacteria with a thick peptidoglycan layer stain gram positive (like staphylococcus aureus) while bacteria with a thin layer stain gram negative (like escherichia coli)
The shape is not specific it could be coccus(spiral), spirila, or bacillus(rod), depends on the bacteria. Gram positives are more complex and need different antibiotics to get rid of them, like tetracycline
Gram positive bacteria protect themselves using antibiotic resistance and their flagella to move. They do not have a membrane like the gram negative bacteria.
The bacteria present in curd are primarily Gram-positive bacteria. These include species like Lactobacillus acidophilus and Streptococcus thermophilus. Gram-negative bacteria are not typically found in curd as the fermentation process favors the growth of Gram-positive bacteria.
gram staining differentiates between gram negative and gram positive bacteria by showing different colors. it shows blue or purple like color for gram positive bacteria and red color for gram negative bacteria. where as simple stain gives the same color to all the types of bacteria. hence it is difficult to differentiate between them.
AnswerYou can use a "selective" medium that will inhibit the growth of Gram positive bacteria and only allow Gram negative bacteria to multiply. A medium which is commonly used for this is the McConkey agar which contains a crystal violet strain and allows only Gram negative cultures to grow.You can also eliminate Gram positive bacteria with antibiotics (e.g. ampicillin) provided that they are sensitive and not resistant.
The gram positive cell wall contains a thicker layer of peptydoglacans than that of gram negative. the gram negative bacteria doesn't have a LPS(Lipopolysaccharides) covering outside the cell wall like the gram negatives.their is a periplasmal space between peptidoglycan cell wall and the cell membrane. During Gram's staining the CVI complex doesnt leave the gram positive bacteria because of the thick layer of peptydoglycanes.
Gram staining is a simple staining test that simply identifies the two main groups of bacteria. Gram positive, and gram negative. Down a microscope, gram pos look like a dark blue/purple colour, and gram neg look red. It is to do with what the wall of the bacteria comprises of, and without going into too much detail, certain drugs work on gram pos bacteria, and others wont. Likewise for gram neg.
Bacteria are classified in many ways. Most bacteria are classified by their shape, cell wall, movement, eating, and breathing. The most useful technique of classification would be the Gram stain. The Gram stain distinguishes between differences in the cell wall composition of bacteria (Gram positive or Gram negative). The shape of the bacteria could be spherical (coccus), rod (bacillus), spirochete, or vibrio (curved rod). The texture could be wet/moist, dry, or mucoid. Bacteria come in a wide variety of colors.
Structures of bacteria: Flagella PiliSex pilus Common pili or fimbriae Capsules Cell wallGram-positive bacteria Gram-negative bacteria Plasma membrane Ribosomes Inclusions Chromosome Plasmid
Coccus or cocci
To determine which pathogen you are dealing with. Bacteria with a thick peptidoglycan layer stain gram positive (like staphylococcus aureus) while bacteria with a thin layer stain gram negative (like escherichia coli)
EDP pathway occurs in cytoplasmic matrix of soil microbes like pseudomonas , rhizobium , acetobacter , agrobacterium and gram negative bacteria. a gram positive bacteria , enterococcus faecalis also uses this pathway.