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Can you elimintate gram positive bacteria from a gram positive gram negative mixture with mild detergent treatment?
Wiki User
∙ 15y ago
A medium which is commonly used for this is the McConkey agar which contains a crystal violet strain and allows only Gram negative cultures to grow.
You can also eliminate Gram positive bacteria with antibiotics (e.g. ampicillin) provided that they are sensitive and not resistant.
Wiki User
∙ 13y ago
Wiki User
∙ 9y agoGram positive selective media like Aztreonam selective agar, phenylethyl alcohol blood agar, esculin-mannitol agar, and Columbia CN blood agar etc. Finding a medium that works for your specific specimen being used will help with separating the gram negative from the gram positive.
Wiki User
∙ 15y agoyes
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How Can you elimintate gram positive bacteria from a gram positive gram negative mixture with mild detergent treatment?
as gram negative have low content of phospholipids than positive detergents at low concentration effectively kill negative bacteria
Will treatment with transpeptidase eliminate gram positive and gram negative bacteria?
no
Can treatment with Transpeptidase eliminate gram positive bacteria from a mixture of gram positive bacteria and gram negative bacteria?
No. A treatment of ethylenediaminetetraacetic acid (EDTA) and the enzyme, lysozyme, will cause gram negative bacteria to lyse. Some gram positive bacteria can also be lysed by doing this. The EDTA stabilizes the solution and allows lysozyme to cut the bonds between the carbohydrates that make up the cell wall wall in bacteria. This will lyse the cell and cause the contents of the cell to be spilled out. This technique is primarily used for DNA assays. A good way to isolate either gram positive or gram negative is by using different types of agar. The MacConkey agarmight be the most useful for what you want to do. It will only allow gram negative to grow, isolating just the gram negatives only.
Are most pathogenic bacteria gram-negative?
Most Pathogenic bacteria are gram negative
What is the difference between positive gram and negative gram?
The major difference between gram positive and the gram negative bacteria is the structure of their cell wall. The gram positive bacteria have two layers a plasma membrane and outer to it a peptidoglycan layer. But the gram negative bacteria have another layer other than the cell wall. While the gram negative bacteria has three layers. It has a plasma membrane that is called as the inner membrane, then a peptidoglycan layer and finally an outer membrane of phospholipids and lipopolysaccharides. There is space between the inner membrane and the peptidoglycan layer called as the periplasmic space. Peptidoclycan layer in the gram positive bacteria are much more thicker than that of the gram negative bacteria. The molecule teichoic acid is present in gram positive bacteria and the porin proteins are characteristically present in the gram negative bacteria. Gram positive bacteria usually produce exotoxins and the gram negative bacteria usually produces endotoxins. Gram positive bacteria gives Violet colour to the gram staining and the gram negative bacteria gives pink colour to the gram staining. In gram staining the bacteria is fixed on slides and then they are treated with Crystal Violet. After that they are treated with iodine and then they are decolourised. If the bacteria is gram negative then it shows Violet colour and if it is gram negative then it shows pink colour. Initially both strains show Violet colour after Crystal Violet treatment but on the treatment with iodine and decolourisation the gram negative bacteria loose colour and become pink. This is because in the gram positive bacteria the Violet crystals bind firmly to the peptidoglycan layer and the colour is not removed in later stages. In gram positive bacteria the colour does not penetrate much because of outer membrane and is lost much during decolourisation.
How Can you elimintate gram positive bacteria from a gram positive gram negative mixture with mild detergent treatment?
as gram negative have low content of phospholipids than positive detergents at low concentration effectively kill negative bacteria
Eliminate gram-positive bacteria from a mixture of gram and gram-bacteria which procedure would be best 1treatment with mild detergent or lysozyme?
to eliminate gram positive bacteria from a mixture of grampositive and gram negative bacteria which procedure would be best first treatment with mild detergent or lysoyme
Will treatment with transpeptidase eliminate gram positive and gram negative bacteria?
no
Can treatment with Transpeptidase eliminate gram positive bacteria from a mixture of gram positive bacteria and gram negative bacteria?
No. A treatment of ethylenediaminetetraacetic acid (EDTA) and the enzyme, lysozyme, will cause gram negative bacteria to lyse. Some gram positive bacteria can also be lysed by doing this. The EDTA stabilizes the solution and allows lysozyme to cut the bonds between the carbohydrates that make up the cell wall wall in bacteria. This will lyse the cell and cause the contents of the cell to be spilled out. This technique is primarily used for DNA assays. A good way to isolate either gram positive or gram negative is by using different types of agar. The MacConkey agarmight be the most useful for what you want to do. It will only allow gram negative to grow, isolating just the gram negatives only.
How do you eliminate gram positive from mix with gram negative?
can this be done by using gram differential stain and then lysozme, washing with a mild detergent mechanically to release the dead debris of the lysed G (+) bacteria.
Is klebsiella gram positive or negative?
i believe it is a gram bacteria which causes respiratory illness.
Are most pathogenic bacteria gram-negative?
Most Pathogenic bacteria are gram negative
Does negative ion kills bacteria?
Negative ions supposedly kill some bacteria. There is some debate on how effective this is and how many negative ions you have to have circulating to kill bacteria.
What are the positive and negative aspects about bacteria?
The positive aspect about bacteria is that weak bacteria can be used to prevent or defend against other bacteria that harms the body which is the negative aspect of bacteria.
What diseases are caused by bacteria gram negative?
pneumonia is a disease caused by the gram negative bacteria
What is the difference between positive gram and negative gram?
The major difference between gram positive and the gram negative bacteria is the structure of their cell wall. The gram positive bacteria have two layers a plasma membrane and outer to it a peptidoglycan layer. But the gram negative bacteria have another layer other than the cell wall. While the gram negative bacteria has three layers. It has a plasma membrane that is called as the inner membrane, then a peptidoglycan layer and finally an outer membrane of phospholipids and lipopolysaccharides. There is space between the inner membrane and the peptidoglycan layer called as the periplasmic space. Peptidoclycan layer in the gram positive bacteria are much more thicker than that of the gram negative bacteria. The molecule teichoic acid is present in gram positive bacteria and the porin proteins are characteristically present in the gram negative bacteria. Gram positive bacteria usually produce exotoxins and the gram negative bacteria usually produces endotoxins. Gram positive bacteria gives Violet colour to the gram staining and the gram negative bacteria gives pink colour to the gram staining. In gram staining the bacteria is fixed on slides and then they are treated with Crystal Violet. After that they are treated with iodine and then they are decolourised. If the bacteria is gram negative then it shows Violet colour and if it is gram negative then it shows pink colour. Initially both strains show Violet colour after Crystal Violet treatment but on the treatment with iodine and decolourisation the gram negative bacteria loose colour and become pink. This is because in the gram positive bacteria the Violet crystals bind firmly to the peptidoglycan layer and the colour is not removed in later stages. In gram positive bacteria the colour does not penetrate much because of outer membrane and is lost much during decolourisation.
Which Gran Positive or negative which is more serious?
Gram positive bacteria responds to the Gram stain; gram negative bacteria does not. The two bacteria do not respond to the same antibiotics. Right now the most dangerous bacteria is a gram negative bacteria. That could change.
