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Why is Erbium perchlorate is used in calibration of HPLC?
Erbium has a strong absorption in uv and visible range, It is used in HPlc calibration for the wavelength accuracy verification of the PDA detector.
Why propyl paraben used in HPLC calibration?
propyl paraben being a stable compound with reproducible chromatographic parameters used as a internal standard for the calibration of the HPLC systems
Which are the top commercial uses for HPLC?
HPLC is used to analyze, identify, purify and quantify organic compounds.
What is the use of calculating asymmetry factor in hplc?
it is used to check the efficiency of the chromatography column used
What is the function of pump in HPLC?
A high-performance liquid chromatography, or HPLC, refers to a technique in analytic chemistry that is used to separate the components in a mixture. The pump in HPLC passes a pressurized liquid solvent that contains the sample mixture through a column filled with a solid adsorbent material.
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
Definition of standard in HPLC?
Answer: HPLC standards are an indispensible tool for analytical HPLC applications. They are used to monitor column performance & calibrate detector response.
What is difference bet GLC and HPLC?
GLC has a stationary liquid phase and gas moving phase HPLC had a stationary solid phase and liquid moving phase HPLC is done under high pressure. HPLC can be used for thermally unstable compounds as opposed to GLC HPLC can be used for polar or low volatile compounds as opposed to GLC
What instruments are used in Tasmania?
As Tasmania is one of Australia's states, the instruments used there will be the same as the instruments used throughout Australia. there is a great variety of westernised instruments, as well as instruments from other cultures.
How does one apply the HPLC princple?
The HPLC principle is used to separate liquid mixtures into individual parts. It's frequently used in several field such as medicine and law. HPLC is specifically used in sports to test for performance enhancing drugs in urine.
What instrument is used to measure the length of a room?
there are many instruments used to measure length . measuring tape is used to measure the length of room.
Where can one purchase HPLC detectors?
You can purchase used HPLC detectors and other equipment from the usedhplc website or from the ebay bidding website. Alternatively you can buy HPLC detectors from the equipnet website.
In what ways does the theoritical plate help us in HPLC?
It can tell you about its capacity to separate substances in chromatography. The same is used in distillative separation.
What is assay by HPLC?
HPLC is high-performance liquid chromatography. This method is used for the separation and production of chemicals but also for chemical analysis.
Why is Erbium perchlorate is used in calibration of HPLC?
Erbium has a strong absorption in uv and visible range, It is used in HPlc calibration for the wavelength accuracy verification of the PDA detector.
Why propyl paraben used in HPLC calibration?
propyl paraben being a stable compound with reproducible chromatographic parameters used as a internal standard for the calibration of the HPLC systems
What is dead volume HPLC?
The dead volume in HPLC is 137.45. The dead volume in science is used in retention measurements and also in thermodynamic studies and the abbreviation HPLC stands for High Pressure Liquid Chromatography.
