HPLC is used to analyze, identify, purify and quantify organic compounds.
mixture of enantiomers can be separated by HPLC
Yes, melamine can be detected by HPLC (High Performance Liquid Chromatography). HPLC is a common analytical technique used to separate and quantify compounds in a mixture, including melamine. Detection methods such as UV-Vis spectroscopy or mass spectrometry can be used in conjunction with HPLC to identify and quantify melamine accurately.
GC can give very resolved sharp peaks with short run time compared to hplc. additionally, there is less compatibility issue in setting an MS up to a GC than HPLC
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High-performance liquid chromatography (HPLC) is commonly used by chemists, biochemists, pharmaceutical scientists, and researchers in various fields for separating and analyzing compounds in a mixture. It is a valuable tool in drug development, food analysis, environmental monitoring, and quality control in industries such as pharmaceuticals, food and beverage, cosmetics, and academia.
Chemists are the primary users of the HPLC instruments. High-performance liquid chromatography has uses in a variety of fields including research, manufacturing, medical, and legal.
GLC has a stationary liquid phase and gas moving phase HPLC had a stationary solid phase and liquid moving phase HPLC is done under high pressure. HPLC can be used for thermally unstable compounds as opposed to GLC HPLC can be used for polar or low volatile compounds as opposed to GLC
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
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why RT was shifting & how to RT calculation in HPLC
mixture of enantiomers can be separated by HPLC
"RS-HPLC method" means "Related Substance HPLC Method".
You can purchase used HPLC detectors and other equipment from the usedhplc website or from the ebay bidding website. Alternatively you can buy HPLC detectors from the equipnet website.
In HPLC RRT means Relative Retention Time and RRF is Relative Response Factor
The dead volume in HPLC is 137.45. The dead volume in science is used in retention measurements and also in thermodynamic studies and the abbreviation HPLC stands for High Pressure Liquid Chromatography.
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commercial used is its use which is gained commercially