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What else can I help you with?
Which are the top commercial uses for HPLC?
HPLC is used to analyze, identify, purify and quantify organic compounds.
What type of species can be separated by HPLC but not by gas liquid chromatography?
mixture of enantiomers can be separated by HPLC
Can melamine detect by HPLC?
Yes, melamine can be detected by HPLC (High Performance Liquid Chromatography). HPLC is a common analytical technique used to separate and quantify compounds in a mixture, including melamine. Detection methods such as UV-Vis spectroscopy or mass spectrometry can be used in conjunction with HPLC to identify and quantify melamine accurately.
Why volatile compounds are analysed in gc but not in hplc?
GC can give very resolved sharp peaks with short run time compared to hplc. additionally, there is less compatibility issue in setting an MS up to a GC than HPLC
Why ordinary liquid pump cannot be used in hplc system?
An ordinary liquid pump cannot be used in HPLC systems because HPLC requires pumps that can generate high pressure to push solvents through the column at a constant and precise flow rate. Ordinary pumps do not provide the level of pressure and flow rate control needed for HPLC separations, which can negatively impact the accuracy and reproducibility of chromatographic results. HPLC pumps are specifically designed to handle the high pressures and flow rates required for efficient separations.
What type of profession uses the HPLC instrumentation for printing?
Chemists are the primary users of the HPLC instruments. High-performance liquid chromatography has uses in a variety of fields including research, manufacturing, medical, and legal.
Which are the top commercial uses for HPLC?
HPLC is used to analyze, identify, purify and quantify organic compounds.
What is difference bet GLC and HPLC?
GLC has a stationary liquid phase and gas moving phase HPLC had a stationary solid phase and liquid moving phase HPLC is done under high pressure. HPLC can be used for thermally unstable compounds as opposed to GLC HPLC can be used for polar or low volatile compounds as opposed to GLC
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
Retention time calculation for hplc?
why RT was shifting & how to RT calculation in HPLC
What type of species can be separated by HPLC but not by gas liquid chromatography?
mixture of enantiomers can be separated by HPLC
What is rs-hplc?
"RS-HPLC method" means "Related Substance HPLC Method".
Where can one purchase HPLC detectors?
You can purchase used HPLC detectors and other equipment from the usedhplc website or from the ebay bidding website. Alternatively you can buy HPLC detectors from the equipnet website.
What is RRT and RRF in hplc?
In HPLC RRT means Relative Retention Time and RRF is Relative Response Factor
What is dead volume HPLC?
The dead volume in HPLC is 137.45. The dead volume in science is used in retention measurements and also in thermodynamic studies and the abbreviation HPLC stands for High Pressure Liquid Chromatography.
What are the key differences between normal phase HPLC and reverse phase HPLC in terms of their separation mechanisms and applications?
Normal phase HPLC separates compounds based on their polarity, with the stationary phase being polar and the mobile phase being nonpolar. Reverse phase HPLC separates compounds based on their hydrophobicity, with the stationary phase being nonpolar and the mobile phase being polar. Normal phase HPLC is typically used for separating polar compounds, while reverse phase HPLC is used for separating nonpolar compounds.
What process that uses pressure to separate substances in a solution?
The process you are referring to is likely a type of chromatography, known as high pressure liquid chromatography (HPLC). In HPLC, a liquid mobile phase is passed through a column of stationary phase under high pressure, separating the components of a mixture based on their interaction with the stationary phase.
