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Agarose gel electrophoresis is the easiest and most common method used in biochemistry and molecular biology in separating DNA or RNA molecules according to their size.
Agarose gel electrophoresis is for determining the size of a piece of RNA or DNA. It can be used to determine the culprit of a crime, relatives, even the cause of some diseases, like Mad Cow.
The pore size of an agarose gel is too small to allow the efficient movement of proteins. Therefore, a poly acrylamide gel is used to separate proteins according to size
You use a buffer when making agarose gels so that when the gel is used for electrophoresis, the gel is able to conduct electricity. The buffer contains ions from the buffer salts that will facilitate conduction. that was good
agarose gel electrophoresis
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An agarose is a polymeric cross-linked polysaccharide extracted from the seaweed agar and used to make gels.
Agarose is made from agarose, a polysaccharide from see weeds. Polyacrylamide is made from the synthetic polymerization of acrylamide, which in its monomeric form is a neurotoxin. Based on these structural differences, it could be said that agarose gels have larger 'pores' than polyacrylamide gels meaning that large particles can move more easily in agarose gels since the agarose polymers are larger and pack less densely then an equivalent amount of polyacrylamide. Therefore, agarose is generally used for the electrophoresis of large molecules such as DNA and RNA or speedy separation (low resolution) of small molecules such as proteins. Polyacrylamide is used for the high resolution electrophoresis of small molecules such as proteins.
Agarose gel electrophoresis is the easiest and most common method used in biochemistry and molecular biology in separating DNA or RNA molecules according to their size.
Agarose gel electrophoresis is for determining the size of a piece of RNA or DNA. It can be used to determine the culprit of a crime, relatives, even the cause of some diseases, like Mad Cow.
Agarose is used in gel electrophoresis to separate nucleic acids (like DNA) by size, charge an other physical properties. Gel electrophoresis uses an electrical current to make particles move. For example, DNA is negative, so it'll travel towards to positive electrode of the gel box. Agarose has small pores through which a DNA can travel. Bigger fragments of DNA travel shorter distances, because it takes longer for them to navigate through the pores of the agarose gel. Identically sized pieces of DNA will travel the same distance, which is why you get bands (DNA with loading dye) after you run a a gel.
The pore size of an agarose gel is too small to allow the efficient movement of proteins. Therefore, a poly acrylamide gel is used to separate proteins according to size
Yes
You use a buffer when making agarose gels so that when the gel is used for electrophoresis, the gel is able to conduct electricity. The buffer contains ions from the buffer salts that will facilitate conduction. that was good
agarose gel electrophoresis
The migration of DNA/Protein on the gel (agarose/polyacrylamide) by the influence of electric charge is called gel electrophoresis. It is used to resolve the biomolecules according to their size(mainly) and shape(for proteins)
Agar comes from algae. It is used to culture bacteria and can be eaten by humans as a substitute for gelatin.