Iodine is used to bind the Crystal Violet to the Gram Positive microbes.
It is crystal violet & stains all cells purple.
Violet
Because violet dye is the least expensive.
You don't get a counterstain.
Iodine is used to bind the Crystal Violet to the Gram Positive microbes.
It is crystal violet & stains all cells purple.
Traditional antifungal drugs such as.gentian violet (also known as crystal violet)
In Gram staining procedure during bacterial staining , iodine forms a complex with crystal violet stain which stains Gram positive bacteria blue to violet .
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If the iodine in the Gram's stain was omitted, the crystal violet which enters the cell will not be able to form the Crystal Violet Iodine (CVI) complex. The CVI complex molecule has a larger size than that of Crystal violet molecule and hence Gram +ve bacteria are able to retain the stain. If iodine were omitted, even Gram+ve cells would get decolorised by Alcohol treatment and would be seen as Gram-ve cells.
Iodine is added as a mordant to enhance crystal violet staining by forming a crystal violet-iodine complex.
Yes it is.
Crystal violet is a hexamethyl also known as methyl violet 10B This is much darker than 2B, and often darker than 6B. It is used in biological stainang particularly gram staining together with safranin and iodine. Crystal violet was also used to treat strept throat. Doctors used to swab it on the back of your throat.
Because violet dye is the least expensive.
Violet
The route below violet city.