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BET TEST- BACTERIAL ENDOTOXIN TEST
IT IS ALSO CALLED LAL TEST
LAL- LIMULUS AMOEBOCYTE LYSATE
WHY WE PERFORM BET TEST ?
BACTERIAL ENDOTOXIN TEST IS PERFORM FOR DETECTION THE ENDOTOXIN IN SAMPLE BECAUSE EACH INTRAVESCULAR/ INTRAMUSCULAR INJECTIONS OR INFUSION HAVE ITS ENDOTOXIN LIMIT.
WE PERFORM BET TEST TO CHECK ENDOTOXIN IN SAMPLE WHICH SHOULD BE LESS THAN GIVEN LIMIT.
REAGENTS USED:-
1. LAL- LIMULUS AMOEBOCYTE LYSATE
2. LRW- LAL REAGENT WATER
3. CSE- CONTROL STANDARD ENDOTOXIN
LAL:- IT IS AQUEOUS EXTRACT OF AMOEBOCYTE (WHICH IS BLOOD CELLS OF LIMULUS POLYPHEMUS (HORSESHOE CRAB).
LRW:- IT IS HIGHLY PURIFIED WATER USED FOR ANALYSIS OF BACTERIAL ENDOTOXIN TEST. IT HAS A MINIMUM QUANTITY OF ENDOTOXIN.
CSE:- IT IS EXTRACT OF LIPID A FROM CELL WALL OF GRAM NEGATIVE BACTERIAL (E. COLI)
IT IS USED FOR POSITIVE CONTROL IN THE TEST.
REACTION:- LAL REACTS WITH BACTERIAL ENDOTOXIN PRESENT IN THE SAMPLE AND GIVE A RESULT AS GEL CLOTING FOR POSITIVE TEST AND NO GEL FORMATION AS A NEGATIVE RESULT.
SAMPLE +LAL REAGENTS ---------------------------------- RESULTS
100 microliter + 100 microliter --------------------------- gel cloting / no gel formation
+ ve result / - ve result
THANKS & REGARDS
SANDEEP K. TYAGI
EXECUTIVE
QUALITY CONTROL DEPARTMENT
V.H.B. MEDISCIENCES LTD. RUDRAPUR
MOB.- 9759430015
9258188360
E.mail:- sandeepmicro@rediffmail.com
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