... so that the cell is regulated. If all the genes were swithced on at one time the cell would go haywire and produce excess amounts of proteins not necessarily needed, and possible causing problems within the cell.
every cell does not express every gene.
The answer is encoded in your question.
By limiting the length of time mRNA is available for translation. Source: Biology textbook
Like prokaryotes, eukaryotes must regulate gene expression. This is accomplished primarily by controlling when RNA polymerase binds to the beginning of a gene. This binding cannot take place in eukaryotes without the aid of transcription factor.
Genetic engineering
Muscle cells,Skin cells, and white blood cells.
The answer is encoded in your question.
By limiting the length of time mRNA is available for translation. Source: Biology textbook
Cystic Fibrosis
n the simplest forms of gene therapy, the DNA must be integrated into the genome of the cell (it must become part of the regular DNA that is in the cell) so that it is replicated when the cell divides and passed on to all the daughter cells. In addition, it has to integrate into a part of the DNA so that it can be expressed so that it can make the missing protein. There are other forms of gene therapy for particular diseases where temporary expression therapeutic gene is all that is needed. For example, to make a tumor susceptible to certain drugs to cure the patient. In this case, integration is not needed and the DNA needs only to get into the nucleus so the gene can be expressed.
Working genes have been inserted into defective cells during gene therapy by first isolating a copy of the gene. Next, the working genes are planted onto a cold virus and as the cold virus infects cells, it also begins producing functional CF protein. Since the cells are "cured", they are able to transport chloride ions across their plasma membranes.
Researchers replace a mutated gene with a healthy copy of the gene. The healthy gene must first attach to a chromosome within a patient's cells. Then the gene needs to make the correct type and amount of protein. These techniques are still experimental at this time.
Like prokaryotes, eukaryotes must regulate gene expression. This is accomplished primarily by controlling when RNA polymerase binds to the beginning of a gene. This binding cannot take place in eukaryotes without the aid of transcription factor.
integrins
There are steps you must follow in the making of recombinant DNA such as use crosses to identify donor, clone gene in bacterium, characterize the gene, modify the gene, and reintroduce the gene into donor cells.
In vitro therapy involves injecting the new gene directly into the target tissue where the individual cells must pick it up.
Genes are segments of DNA that code for proteins. Genes are either regulated by being switched "off" or "on" - meaning they are not currently making their proteins OR they are currently making their proteins. It is to a cell's advantage to not make proteins from genes when they are not needed to conserve energy and building materials. Hope this helps - Coach H
The process of replacing a defective gene with a healthy gene is called gene therapy. Gene therapy is done by cutting genes from the DNA of healthy cells and inserting them into the DNA of defective cells and tissues.