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DNA is used to form bonds between the inserted gene and the plasmid DNA?
Chrisfumbanks
ligase
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∙ 14y ago
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What is the bacterial cell called when the f factor is in plasmid form?
F Plasmid
How do bonds form between water molecules?
The bond between water molecules is known as a hydrogen bond.
What two bases form three hydrogen bonds?
cytosine and guanine form three hydrogen bonds between them
How does DNA technology transfer bacterial genes from one cell to another?
Gene transfer between cells generally consists of the following steps: 1. Isolating the gene of interest - Here, the gene which has to be transferred has to be isolated from the genome of the source (or host) organism. 2. Splicing the gene if interest into a plasmid. Splicing is a process wherein a foreign strand of DNA (the gene of interest) is inserted into a loop of DNA called a plasmid. The plasmid DNA is cut open to form a linear fragment. The gene of interest is then attached to the plasmid DNA. The plasmid DNA is converted back into the loop form with the help of an enzyme called DNA ligase. 3. Gene amplification: Here, the plasmid containing the gene of interest is amplified. Which means, many copies of the plasmid containing DNA are created through a process called the polymerase chain reaction. 4. Transfection: This is the final step wherein the plasmid containing DNA is inserted into the recipient organism. Sometimes the foreign DNA remains within the plasmid and is able to express protein. Other times, the gene of interest can be engineered to contain a sequence called the recombination sequence which will allow it to integrate (or join) the host genome through a process called homologous recombination. By the method described above, a foreign gene is removed from one organism and inserted into another. If the gene of interest is integrated into the host of the recipient organism, copies of it are made every time the host cells divide.
Do hydrogen bonds only form between adenine and cytosine?
no, they form between adenine and thymine.
Ionic bonds form between what kinds of elements?
Ionic bonds form between a metal and a non-metal.
What is the bacterial cell called when the f factor is in plasmid form?
F Plasmid
What kind of bonds form between water?
hydrogen bonds
What type of elements do covalent bonds form between?
Rule of thumb answer is covalent bonds form between non metal elements.
What is used to form bonds in an atom?
Bonds between atoms involve electrons.
What kind of bonds do alcohols form between individual molecules?
Hydrogen bonds
How do bonds form between water molecules?
The bond between water molecules is known as a hydrogen bond.
What kinds of bonds form between amino acids to form protein chains?
They are [accurately] termed to be peptide bonds.
What two bases form three hydrogen bonds?
cytosine and guanine form three hydrogen bonds between them
How does DNA technology transfer bacterial genes from one cell to another?
Gene transfer between cells generally consists of the following steps: 1. Isolating the gene of interest - Here, the gene which has to be transferred has to be isolated from the genome of the source (or host) organism. 2. Splicing the gene if interest into a plasmid. Splicing is a process wherein a foreign strand of DNA (the gene of interest) is inserted into a loop of DNA called a plasmid. The plasmid DNA is cut open to form a linear fragment. The gene of interest is then attached to the plasmid DNA. The plasmid DNA is converted back into the loop form with the help of an enzyme called DNA ligase. 3. Gene amplification: Here, the plasmid containing the gene of interest is amplified. Which means, many copies of the plasmid containing DNA are created through a process called the polymerase chain reaction. 4. Transfection: This is the final step wherein the plasmid containing DNA is inserted into the recipient organism. Sometimes the foreign DNA remains within the plasmid and is able to express protein. Other times, the gene of interest can be engineered to contain a sequence called the recombination sequence which will allow it to integrate (or join) the host genome through a process called homologous recombination. By the method described above, a foreign gene is removed from one organism and inserted into another. If the gene of interest is integrated into the host of the recipient organism, copies of it are made every time the host cells divide.
What bonds form between monosaccharides during the formation of a disaccharide?
The bonds that form between two or more monosaccharides during the formation of a disaccharide is a glycosidic linkage.
What is a covalent bonds form?
covalent bonds are formed when electrons are shared between atoms.
