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DNA moleule with length1800bp were treated with cutting enzyme Hae3 and two fragments were produced one a length of 1000bp so how many restricton sites for Hae3 exist inthis DNA and the length of seco?
Wiki User
∙ 16y ago
the restriction endonuclease(HaeIII) has one restriction site on that DNA molecule. If one of the fragment is 1000bp long then the length of other fragment should be 800bp.
Wiki User
∙ 16y ago
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What are cutting DNA at specific sites to form restriction fragments called?
RFLPs
Based on restriction maps of plasmid determine the number of DNA fragments and sizes of the fragments?
Plasmids are circular pieces of DNA, so the number of fragments equals the number of cuts from the restriction enzymes. You can easily see this if you start with one restriction enzyme that cuts the plasmid in only one place. Cutting the circle in one place yields you only one fragment. If the restriction cuts in two places, you end up with two fragments; with three places, three fragments, etc. With linear chromosomes, the situation is different. Cutting a linear chromosome in one place yields two fragments, cutting in two places yields three fragments, etc. So the number of fragments is always one more than the number of cuts. A restriction map of a plasmid will show all of the cuts the restriction enzymes made. Each cut is labeled with the enzyme that made it. One can count the spaces between cuts to determine the number of fragments that are produced. Restriction maps usually (but not always) also show the size of each fragment.
Where does the wastage of diamond goes?
Diamond fragments separated from a diamond stone during the cutting process are collected and used to cut and polish other diamonds.
Why was the discovery of restriction enzymes important to recombinant DNA technology?
Recombinant DNA technology requires fragments of DNA from the source genome. Using crude methods such as mechanical shearing, we get random fragments of DNA, and their sequence is unknown. Restriction enzymes are specific in site recognition and cutting and their discovery lead to proper fragments of DNA which have some known sequences.
What process is used to cut DNA into fragments?
DNA can be fragmented using restriction endonucleases or restriction enzymes. Restriction enzymes identify specific sequences within the DNA and cause cleavage generating fragments. When this digested DNA is allowed to run in gel electrophoresis fragments get separated according to their mass. When visualized under UV transilluminator, fragmented DNA can be observed as fluorescing bands.
What is a term for a shape produced by cutting through a 3D object?
cross section
What does the term sticky ends refer to in gene splicing?
Sticky ends are produced by cutting the DNA in a staggered manner within the recognition site producing single-stranded DNA ends. These ends have identical nucleotide sequence and are sticky because they can hydrogen-bond to complementary tails of other DNA fragments cut by the same restriction enzyme.
What is the definition of nicking?
of Nick, The cutting made by the hewer at the side of the face., Small coal produced in making the nicking.
How is chenille fabric produced?
Chenille fabric is produced by weaving chenille "yarn" into new cloth. The chenille "yarn" is produced by first weaving a specific type of fabric and cutting that fabric into strips, which are then woven up into the chenille fabric.
What tools of recombinant DNA technology is incorrectly paired with its use?
DNA polymerase-polymerase chain reaction to amplify sections of DNA reverse transcriptase-production of cDNA from mRNA DNA ligase-cutting DNA, creating sticky ends of restriction fragments restriction enzyme-analysis of RFLPs electrophoresis-separation of DNA fragments
Why are leaf-cutters also known as parasol ants?
After cutting out pieces of leaves, the ants carry them away holding the pieces over themselves, almost as if the leaf fragments are parasols, protecting the ants from the sunlight.
Why are leaf ants also known as parasol ants?
After cutting out pieces of leaves, the ants carry them away holding the pieces over themselves, almost as if the leaf fragments are parasols, protecting the ants from the sunlight.
