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Not in general replication as the leading strand is replicated straight away. The lagging strand is replicated in pieces that need ligase to affix them, one to the other.

However, in a replication bubble, when a lagging strand runs into a leading strand, and later the primers are replaced, ligase is still needed. Therefore, technically, the leading strand does need the ligase once.

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8y ago
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11y ago

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Q: Does dna ligase help assemble the leading strand?
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