Yes, the readings usually follow the plan: Old Testament, Psalm, New Testament (Epistle or other non-Gospel reading), Alleluia verse, Gospel in a Sunday Mass. The Second reading is omitted during the week, and the first reading may be from either the Old Testament or the New Testament.
The Bible is used in Mass during the Liturgy of the Word. An Old Testament reading, some psalms, a New Testament reading and a Gospel reading all come from the Bible at this point of the mass. At other points in the mass there are references that come from the Bible, like at the consecration and some prayers.
The New Testament is often read during the Epistle and always at the Gospel. Many prayers of the Mass also come from the New Testament, as does the very structure of the Mass.
The Second Reading generally comes from one of the books in the New Testament, not including the Gospels. Usually it comes from an epistle, or a letter, written to the early Christians by St. Paul. This is because the epistles make up the good majority of the New Testament.
It is always from the New Testament. The first reading is usually ( but not always) from the Old Testament (Hebrew Scriptures)
Usually the Old Testament.
The Old Testament
In the Catholic Church, there are two different cycles of readings, one for Sunday Masses (allowing the Church to read nearly the entire Bible in three years on Sundays) and one for weekday Masses (which covers the same amount of the Bible in two years). It is important to remember that Sunday Masses have three readings while weekday Masses have two. During Sunday Masses, the first reading usually comes from the Old Testament, with rare exceptions including the Sundays of the Easter Season, when the first readings come from the Book of Acts. Also, during Advent, the first reading comes from the book of Revelation on Sundays. During weekday Masses, the first reading comes from either the Old or New Testament.
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There are normally two readings at Mass during the "Mass of the Catechumens" or instructional first half of Mass. They are properly the Epistle and the Gospel. This question is asking about the Epistle. Traditionally, in the Catholic Church, the Epistle was more often taken from St. Paul's epistles than the Old Testament. The Epistle was to give moral encouragement and instruction on virtue and Christian living. During the liturgical reforms of the 1960s, it was decided that readings would more often be taken from the Old Testament, to better mirror the Hebraeic origins of the Mass as well as connect the old with the new, which would follow in the Gospel. Thus, presently, it is rather arbitrary if the Epistle is taken from the Old Testament or the New, since some readings remain unmodified from the liturgical practices predating the 1960s liturgical reforms. If the topic interests you, it might be worth buying or finding a missal predating 1960 and comparing it to the present day missal, to see which readings remain the same. Also, during the Easter Season the first reading (Old Testament) is usually taken from the Acts of the Apostles (New Testament) so all the readings come from the New Testament.
Roman Catholic AnswerThe second reading usually comes from the Epistles, or from Revelation.
Before James in the New Testament is Hebrews and after James is 1 Peter.
In the Catholic Church, there are two different cycles of readings, one for Sunday Masses (allowing the Church to read nearly the entire Bible in three years on Sundays) and one for weekday Masses (which covers the same amount of The Bible in two years). It is important to remember that Sunday Masses have three readings while weekday Masses have two. During Sunday Masses, the first reading usually comes from the Old Testament, with rare exceptions including the Sundays of the Easter Season, when the first readings come from the Book of Acts. Also, during Advent, the first reading comes from the book of Revelation on Sundays. During weekday Masses, the first reading comes from either the Old or New Testament.