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RIA measures scintillation due to decay of radioactive tracer. ELISA measures a color change due to an enzyme tracer.
No is stays clear
Black
the color is rainbow or black
PX8 - Black, Clear Coat
Yellow and black represents change.
Go to Properties and then go to color
There needs to be a conversion of the substrate for the chemical reaction to provide an H+ ion for the color change needed for ELISA. The temperature essentially lowers the activation energy to allow for this reaction to proceed.
Baby black panthers change their fur color as its a adult
So that when the substrate is added, the reaction between the enzyme and the substrate will cause a change in color
In the Indirect ELISA ,An antigen is added to the microtiter plate well and the antigen attaches to the walls of the microtiter plate.After rinsing to remove excess antigen, the serum suspected of containing the antibodies is added.Enzyme-linked antibody capable of reacting with the constant region of other antibodies is the added, followed by addition of the colorless substrate. Development of color indicates the presence of the antibody being identified.
black